The Comparative Molecular Epidemiology of GII.P7_GII.6 and GII.P7_GII.7 Norovirus Outbreaks in Victoria, Australia, 2012-2014
| Autor: | Jean M. Moselen, John Marshall, Leesa D. Bruggink |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Genotype viruses Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Disease Outbreaks Feces Open Reading Frames 03 medical and health sciences fluids and secretions Phylogenetics Virology medicine Humans Phylogeny Caliciviridae Infections Molecular epidemiology Incidence Incidence (epidemiology) Norovirus Age Factors Australia virus diseases Outbreak Sequence Analysis DNA Middle Aged Gastroenteritis 030104 developmental biology Infectious Diseases RNA Viral |
| Zdroj: | Intervirology. 59:60-65 |
| ISSN: | 1423-0100 0300-5526 |
| Popis: | The comparative molecular epidemiology of the related GII.P7_GII.6 and GII.P7_GII.7 noroviruses has not been examined in detail. ORF 1, ORF 2 and ORF 1/ORF 2 RT-PCR as well as sequencing and phylogeny analysis were carried out on faecal specimens from 873 gastroenteritis outbreaks in Victoria, Australia (2012-2014). There were 575 (66%) detected as positive for norovirus by means of ORF 1 RT-PCR and/or ORF 2 RT-PCR. Of these, 24 (4.2%) were GII.6 (ORF 2) outbreaks, 7 (1.2%) were GII.7 (ORF 2) outbreaks, and 1 outbreak (0.2%) involved both GII.6 (ORF 2) and GII.7 (ORF 2) noroviruses. The median age of patients identified with GII.6 (ORF 2) (84 years) was significantly different from that of patients identified with GII.7 (ORF 2) (39 years). ORF 2 GII.6 and ORF 2 GII.7 sequences were always associated with a GII.P7 ORF 1 sequence, and GII.P7 sequences fell into two clusters, with one corresponding to the GII.6 ORF 2 genotype and the other to the GII.7 ORF 2 genotype, thereby indicating that the ORF 1 has been evolving separately for the two viruses. Thus, two closely related noroviruses can have a markedly different incidence and epidemiology. |
| Databáze: | OpenAIRE |
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