Genetic mapping uncovers cis-regulatory landscape of RNA editing
| Autor: | Jin Billy Li, Mary Anna Carbone, Trudy F. C. Mackay, Patricia Deng, Gokul Ramaswami, Rui Zhang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adenosine Deaminase
Quantitative Trait Loci General Physics and Astronomy Regulatory Sequences Ribonucleic Acid Biology Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Animals Drosophila Proteins Guide RNA RNA Double-Stranded 030304 developmental biology Genetics 0303 health sciences Multidisciplinary Intron Chromosome Mapping RNA General Chemistry biology.organism_classification RNA silencing Drosophila melanogaster RNA editing Regulatory sequence ADAR RNA Editing 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Adenosine-to-inosine (A-to-I) RNA editing, catalysed by ADAR enzymes conserved in metazoans, plays an important role in neurological functions. Although the fine-tuning mechanism provided by A-to-I RNA editing is important, the underlying rules governing ADAR substrate recognition are not well understood. We apply a quantitative trait loci (QTL) mapping approach to identify genetic variants associated with variability in RNA editing. With very accurate measurement of RNA editing levels at 789 sites in 131 Drosophila melanogaster strains, here we identify 545 editing QTLs (edQTLs) associated with differences in RNA editing. We demonstrate that many edQTLs can act through changes in the local secondary structure for edited dsRNAs. Furthermore, we find that edQTLs located outside of the edited dsRNA duplex are enriched in secondary structure, suggesting that distal dsRNA structure beyond the editing site duplex affects RNA editing efficiency. Our work will facilitate the understanding of the cis-regulatory code of RNA editing. Adenosine-to-inosine (A-to-I) RNA editing plays an important role in neurological functions. Here, by a quantitative trait loci (QTL) mapping approach in 131 Drosophila melanogaster strains, the authors identify 545 QTLs associated with differences in RNA editing. |
| Databáze: | OpenAIRE |
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