Longitudinal Imaging of Liver Cancer Using MicroCT and Nanoparticle Contrast Agents in CRISPR/Cas9-Induced Liver Cancer Mouse Model
| Autor: | Sung Dae Kim, Sang Bu An, Kwangmo Yang, Eunji Kim, Chang Won Kim, Si Ho Choi, Jae Soo Koh |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
longitudinal imaging Carcinogenesis mouse model media_common.quotation_subject Contrast Media liver cancer Mice 03 medical and health sciences Liver Neoplasms Experimental 0302 clinical medicine nanoparticle contrast agents Animals Medicine CRISPR Contrast (vision) Longitudinal Studies CRISPR/Cas9 RC254-282 030304 developmental biology media_common 0303 health sciences microCT business.industry Neoplasms. Tumors. Oncology. Including cancer and carcinogens X-Ray Microtomography Longitudinal imaging medicine.disease Mice Inbred C57BL Oncology 030220 oncology & carcinogenesis Time course Cancer research Nanoparticles Original Article CRISPR-Cas Systems business Liver cancer Plasmids |
| Zdroj: | Technology in Cancer Research & Treatment Technology in Cancer Research & Treatment, Vol 20 (2021) |
| ISSN: | 1533-0338 1533-0346 |
| DOI: | 10.1177/15330338211016466 |
| Popis: | Introduction: Micro-computed tomography with nanoparticle contrast agents may be a suitable tool for monitoring the time course of the development and progression of tumors. Here, we suggest a practical and convenient experimental method for generating and longitudinally imaging murine liver cancer models. Methods: Liver cancer was induced in 6 experimental mice by injecting clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats-associated protein 9 plasmids causing mutations in genes expressed by hepatocytes. Nanoparticle agents are captured by Kupffer cells and detected by micro-computed tomography, thereby enabling longitudinal imaging. A total of 9 mice were used for the experiment. Six mice were injected with both plasmids and contrast, 2 injected with contrast alone, and one not injected with either agent. Micro-computed tomography images were acquired every 2- up to 14-weeks after cancer induction. Results: Liver cancer was first detected by micro-computed tomography at 8 weeks. The mean value of hepatic parenchymal attenuation remained almost unchanged over time, although the standard deviation of attenuation, reflecting heterogeneous contrast enhancement of the hepatic parenchyma, increased slowly over time in all mice. Histopathologically, heterogeneous distribution and aggregation of Kupffer cells was more prominent in the experimental group than in the control group. Heterogeneous enhancement of hepatic parenchyma, which could cause image quality deterioration and image misinterpretation, was observed and could be due to variation in Kupffer cells distribution. Conclusion: Micro-computed tomography with nanoparticle contrast is useful in evaluating the induction and characteristics of liver cancer, determining appropriate size of liver cancer for testing, and confirming therapeutic response. |
| Databáze: | OpenAIRE |
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