Microscopic and PCR-based detection of microsporidia spores in human stool samples
| Autor: | Laura Cervi, Patricia Catalina Paulin, Silvana Carnevale, Lorena Guasconi, J. D. Arneodo, Ignacio Beccacece, Verónica Liliana Burstein, Gonzalo Castro, Germán Astudillo, Cristian Javier Mena, Laura S. Chiapello, Martín G. Theumer, Andrés Barnes |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Microsporidiosis Polymerase Chain Reaction Microbiology Feces fluids and secretions parasitic diseases Genotype Microspora medicine Humans Enterocytozoon bieneusi biology Obligate fungi virus diseases General Medicine Enterocytozoon Spores Fungal biology.organism_classification medicine.disease Virology Microsporidia Nested polymerase chain reaction |
| Zdroj: | Revista Argentina de Microbiología. 53:124-128 |
| ISSN: | 0325-7541 |
| DOI: | 10.1016/j.ram.2020.04.005 |
| Popis: | Microsporidia are obligate intracellular fungi with a remarkable ability to infect a wide range of invertebrate and vertebrate hosts. Namely, Enterocytozoon bieneusi is the most frequently microsporidia reported worldwide, and mainly associated with chronic diarrhea and wasting syndrome in AIDS patients. Microscopy and PCR-based detection techniques are effective for diagnosis and identification of species and genotypes; however, these methods should be standardized in each laboratory. In this study, we performed microscopy and nested PCR techniques with PCR product sequencing to detect E. bieneusi in human stool samples. These techniques, if applied together, might prove useful for diagnosis and future epidemiological studies of intestinal microsporidiosis in Argentina. |
| Databáze: | OpenAIRE |
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