The Next Generation of IR Spectroscopy: EC-QCL-Based Mid-IR Transmission Spectroscopy of Proteins with Balanced Detection
Autor: | Andreas Schwaighofer, Mateusz Żbik, Bernhard Lendl, Artur Trajnerowicz, Christopher Karim Akhgar, Jarosław Pawluczyk, Georg Ramer |
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Rok vydání: | 2020 |
Předmět: |
Spectrophotometry
Infrared Analytical chemistry Infrared spectroscopy 010402 general chemistry 01 natural sciences Protein Structure Secondary Article Analytical Chemistry law.invention Hemoglobins chemistry.chemical_compound law Amide Concanavalin A Animals Spectroscopy Absorption (electromagnetic radiation) Detection limit Aqueous solution Chemistry 010401 analytical chemistry Fabaceae Laser 0104 chemical sciences Transmission (telecommunications) Cattle gamma-Globulins Lasers Semiconductor |
Zdroj: | Analytical Chemistry |
ISSN: | 0003-2700 |
DOI: | 10.1021/acs.analchem.0c01406 |
Popis: | We report a mid-IR transmission setup for the analysis of the protein amide I and amide II band in aqueous solutions that achieves a limit of detection as low as 0.0025 mg mL–1 (outperforming our previous results and other state-of-the-art mid-IR-based techniques by almost an order of magnitude). This large improvement is made possible by combining the latest-generation external cavity-quantum cascade laser (EC-QCL) operated at room temperature with an optimized double-beam optical setup that adjusts the path length (26 μm) to ensure robust sample handling. For minimizing the noise introduced by the high-intensity laser light source, a thermoelectrically cooled mercury cadmium telluride balanced detection module was employed. In this way, noise levels better by a factor of up to 20 were achieved compared with single-channel measurements. Characteristic spectral features of proteins with different secondary structures were successfully identified at concentrations as low as 0.1 mg mL–1. Furthermore, a highly linear response was demonstrated for concentrations between 0.05 and 10 mg mL–1. The total acquisition time of the setup can be adapted to fulfill the required sensitivity of the protein measurements and to ensure maximum flexibility for future applications. The presented setup combines high sensitivity, large optical path lengths, and short measurement times and thus outperforms previous research type EC-QCL setups as well as commercially available instruments. This opens a wide range of future applications including protein–ligand interaction studies as well as qualitative and quantitative analyses of proteins in complex matrices such as those found in up- and downstream bioprocess monitoring and similar challenging applications which can not be readily met by conventional FT-IR spectroscopy. |
Databáze: | OpenAIRE |
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