RAZVOJ HOMOZIGOTNIH LINIJA KUKURUZA POMOĆU INDUKCIJE HAPLOIDA IN VIVO U HRVATSKOJ GERMPLAZMI
| Autor: | Domagoj Šimić, Ivan Brkić, Antun Jambrović, Sonja Vila, Maja Mazur |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Germplasm
Genetics Croatian Biology lcsh:Plant culture in vivo haploid induction doubled haploid lines R-nj marker system chromosome doubling colchicine lcsh:S1-972 language.human_language In vivo language lcsh:SB1-1110 Ploidy lcsh:Agriculture (General) Agronomy and Crop Science indukcija haploida in vivo udvostručene haploidne linije sustav markera R-nj udvostručenje kromosoma kolhicin |
| Zdroj: | Poljoprivreda, Vol 25, Iss 1, Pp 19-25 (2019) Poljoprivreda Volume 25 Issue 1 |
| ISSN: | 1848-8080 1330-7142 |
| Popis: | The in vivo haploid induction has been widely applied to the maize breeding in recent decades, but it has not been used in the breeding programs in the Republic of Croatia by now. This study's objectives were to examine the haploid induction rates in the Croatian germplasm and to evaluate the properties of the D0 haploids, which are essential for a successful implementation of this method in breeding. The in vivo haploid induction was performed on 11 single-cross hybrids using the Zarodyshevy Marker Krasnodarsky (ZMK) inducer, and colchicine was used as a chromosome doubling agent. Emergence, misclassification rate, colchicine treatment survival, chromosome doubling rate and self-pollination success were examined in the D0 generation. The haploid induction rates ranged from 6.9 to 15.8%, which is consistent with the average induction rates characteristic of the ZMK inducer and the other modern ones. Significant differences were found among the populations of D0 haploids for all tested properties, except for self-pollination success. On average, the misclassification rates were lower, and the seedling survival rates were higher than those reported in other studies, indicating a possibility of a successful application of the doubled haploid method in maize breeding. Indukcija haploida in vivo posljednjih se desetljeća sve više primjenjuje u oplemenjivanju kukuruza, ali se dosad nije koristila u oplemenjivačkim programima u Republici Hrvatskoj. Ciljevi ovoga istraživanja bili su utvrditi relativne frekvencije induciranih haploida kod germplazme koja se upotrebljava u domaćim oplemenjivačkim programima i ispitati svojstva haploida generacije D0 koja su važna za uspješnu primjenu ove metode u oplemenjivanju. Indukcija haploida in vivo obavljena je na 11 jednostrukih hibrida pomoću induktora ZMK, a za udvostručenje kromosoma korišten je kolhicin. U generaciji D0 ispitana su svojstva nicanja, pogrešne klasifikacije, preživljavanja tretmana kolhicinom, udvostručenja kromosoma i uspješnosti izvođenja samooplodnje. Relativna frekvencija haploida kretala se od 6,9 do 15,8%, što je usporedivo s prosječnim stopama indukcije specifičnim za induktor ZMK i druge moderne induktore. Statistički značajne razlike pronađene su između populacija haploida D0 za sva ispitana svojstva, osim za uspješnost izvođenja samooplodnje. U prosjeku, stope pogrešne klasifikacije bile su niže, a stope preživljavanja tretmana kolhicinom više u odnosu na one zabilježene u drugim istraživanjima, što ukazuje na mogućnost uspješne primjene metode udvostručenih haploida u oplemenjivanju. |
| Databáze: | OpenAIRE |
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