Synthesis and Electron Paramagnetic Resonance Studies of Oligodeoxynucleotides Containing 2-N-tert-Butylaminoxyl-2′-deoxyadenosines
| Autor: | John Walter Mirc, Kazuteru Usui, Mariko Aso, Manami Kurita, Hiroshi Suemune, Shintaro Matsumoto, Yoshitaka Higuchi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Analytical chemistry
010402 general chemistry 01 natural sciences Biochemistry Spectral line Nucleobase law.invention chemistry.chemical_compound Deoxyadenosine law Electron paramagnetic resonance Molecular Biology Base Sequence Deoxyadenosines 010405 organic chemistry Chemistry Organic Chemistry Electron Spin Resonance Spectroscopy Nucleic Acid Hybridization hemic and immune systems respiratory system 0104 chemical sciences Crystallography Oligodeoxyribonucleotides Duplex (building) Nucleic acid Molecular Medicine Spin Labels |
| Zdroj: | ChemBioChem. 17:2346-2352 |
| ISSN: | 1439-4227 |
| Popis: | Oligodeoxynucleotides (ODNs) containing 2-N-tert-butylaminoxyl-2'-deoxyadenosine (A*) residues were synthesized to allow accurate monitoring of adenine motion by EPR spectroscopy through the agency of direct linkage of the acyclic aminoxyl group to the nucleobase, and EPR studies of the ODNs in single- and double-stranded forms were performed. Upon duplex formation, peak broadening and decreases in peak height were observed in EPR spectra, and the synthesized ODNs were shown to be excellent monitors of hybridization. Comparison of peak height and the h1 /h0 signal ratio provided information on the relative mobility of A* in duplexes with different stability. A second set of ODNs each containing two A* residues at different intervals and four dA residues were also synthesized. For these ODNs, correlations were observed between the EPR spectral shapes of the duplexes and the number of dA residues between A* residues, thus demonstrating the potential of A* residues in monitoring of the structures of nucleic acids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text