MAGI-1 PDZ2 Domain Blockade Averts Adenovirus Infection via Enhanced Proteolysis of the Apical Coxsackievirus and Adenovirus Receptor
| Autor: | S. Dean Rider, Katherine J. D. A. Excoffon, Mahmoud S. Alghamri, Timothy L. Williamson, Abimbola O. Kolawole, Heather A. Hostetler, Priyanka Sharma, James M. Readler, Ran Yan, David R. Cool |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
Coxsackie and Adenovirus Receptor-Like Membrane Protein viruses Adenoviridae Infections Immunology PDZ domain Coxsackievirus ADAM17 Protein 01 natural sciences Microbiology Regulated Intramembrane Proteolysis Adenoviridae Madin Darby Canine Kidney Cells 03 medical and health sciences Transduction (genetics) Mice Dogs Protein Domains Virology medicine Animals Humans Adenovirus infection Receptor 030304 developmental biology Adaptor Proteins Signal Transducing 0303 health sciences biology 010405 organic chemistry virus diseases 3T3 Cells biology.organism_classification medicine.disease 0104 chemical sciences Cell biology Virus-Cell Interactions HEK293 Cells Insect Science Cell-penetrating peptide Amyloid Precursor Protein Secretases Cell Adhesion Molecules Guanylate Kinases Binding domain |
| Zdroj: | J Virol |
| ISSN: | 1098-5514 |
| Popis: | Adenoviruses (AdVs) are etiological agents of gastrointestinal, heart, eye, and respiratory tract infections that can be lethal for immunosuppressed people. Many AdVs use the coxsackievirus and adenovirus receptor (CAR) as a primary receptor. The CAR isoform resulting from alternative splicing that includes the eighth exon, CAR(Ex8), localizes to the apical surface of polarized epithelial cells and is responsible for the initiation of AdV infection. We have shown that the membrane level of CAR(Ex8) is tightly regulated by two MAGI-1 PDZ domains, PDZ2 and PDZ4, resulting in increased or decreased AdV transduction, respectively. We hypothesized that targeting the interactions between the MAGI-1 PDZ2 domain and CAR(Ex8) would decrease the apical CAR(Ex8) expression level and prevent AdV infection. Decoy peptides that target MAGI-1 PDZ2 were synthesized (TAT-E6 and TAT-NET1). PDZ2 binding peptides decreased CAR(Ex8) expression and reduced AdV transduction. CAR(Ex8) degradation was triggered by the activation of the regulated intramembrane proteolysis (RIP) pathway through a disintegrin and metalloproteinase (ADAM17) and γ-secretase. Further analysis revealed that ADAM17 interacts directly with the MAGI-1 PDZ3 domain, and blocking the PDZ2 domain enhanced the accessibility of ADAM17 to the substrate (CAR(Ex8)). Finally, we validated the efficacy of TAT-PDZ2 peptides in protecting the epithelia from AdV transduction in vivo using a novel transgenic animal model. Our data suggest that TAT-PDZ2 binding peptides are novel anti-AdV molecules that act by enhanced RIP of CAR(Ex8) and decreased AdV entry. This strategy has additional translational potential for targeting other viral receptors that have PDZ binding domains, such as the angiotensin-converting enzyme 2 receptor. IMPORTANCE Adenovirus is a common threat in immunosuppressed populations and military recruits. There are no currently approved treatments/prophylactic agents that protect from most AdV infections. Here, we developed peptide-based small molecules that can suppress AdV infection of polarized epithelia by targeting the AdV receptor, coxsackievirus and adenovirus receptor (CAR(Ex8)). The newly discovered peptides target a specific PDZ domain of the CAR(Ex8)-interacting protein MAGI-1 and decrease AdV transduction in multiple polarized epithelial models. Peptide-induced CAR(Ex8) degradation is triggered by extracellular domain (ECD) shedding through ADAM17 followed by γ-secretase-mediated nuclear translocation of the C-terminal domain. The enhanced shedding of the CAR(Ex8) ECD further protected the epithelium from AdV infection. Taken together, these novel molecules protect the epithelium from AdV infection. This approach may be applicable to the development of novel antiviral molecules against other viruses that use a receptor with a PDZ binding domain. |
| Databáze: | OpenAIRE |
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