Melanoma vasculogenic mimicry is strongly related to reactive oxygen species level
| Autor: | E V Stepanova, Mikhail Lichinitser, Amalia Vartanian, O. S. Burova, Anatoly Y. Baryshnikov |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Vascular Endothelial Growth Factor A
Cancer Research Angiogenesis Inhibitors Apoptosis Dermatology Biology Resveratrol Antioxidants Mice chemistry.chemical_compound Western blot Cell Line Tumor Stilbenes medicine Animals Humans Vasculogenic mimicry Melanoma chemistry.chemical_classification Reactive oxygen species Neovascularization Pathologic medicine.diagnostic_test Caspase 3 Cytochromes c medicine.disease Capillaries Mice Inbred C57BL Vascular endothelial growth factor Oncology chemistry Cell culture Immunology Cancer research Female Reactive Oxygen Species |
| Zdroj: | Melanoma Research. 17:370-379 |
| ISSN: | 0960-8931 |
| Popis: | The concept of 'vasculogenic mimicry' (VM) was introduced to describe the unique ability of highly invasive tumor cells to form capillary-like structures (CLS) and matrix-rich patterned network in three-dimensional culture that mimic embryonic vasculogenic network. Recently, we have shown that CLS formation requires apoptotic cell death through activation of caspase-3-dependent mechanism. In this study, to identify some molecular determinants driving aggressive melanoma cells to express a latent 'angiogenic program' that recapitulates the early events of CLS formation, we focused on the involvement of antioxidants (AOs) in the process of melanoma VM. We have studied the effects of resveratrol, (-)-epigallocathechin gallate, N-acetyl-cysteine (NAC) and Trolox on the ability of melanoma cells to form/destroy CLS. We observed that the formation of CLS was strongly related to reactive oxygen species level. In vivo animal experiments confirmed the involvement of reactive oxygen species level in melanoma VM. To understand the molecular mechanisms of this phenomenon, we specifically looked for induction of apoptosis and vascular endothelial growth factor (VEGF) release. Western blot analysis revealed that the level of VEGF, VEGF receptors (VEGF-Rs) and active caspase-3 dramatically decreased in cells treated with AOs. Here, we also report further experiments designed to determine whether the crosstalk between AOs and apoptosis exists in melanoma VM. |
| Databáze: | OpenAIRE |
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