In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen
| Autor: | Klaus Dieter Hinsch, Katja Bogner, Elvira Hinsch, Susanne Mueller-Schloesser, Frank Mueller-Schloesser, V. A. Aires |
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| Rok vydání: | 2003 |
| Předmět: |
Male
endocrine system Cryoprotectant Acrosome reaction Semen Insemination law.invention Andrology fluids and secretions Food Animals law Animals Small Animals Sperm motility Cryopreservation urogenital system Equine Chemistry Extender Sperm Egg Yolk Semen extender Phosphatidylcholines Sperm Motility Animal Science and Zoology Cattle Soybeans Acrosome Sperm Capacitation Semen Preservation |
| Zdroj: | Theriogenology. 60(2) |
| ISSN: | 0093-691X |
| Popis: | Semen diluents containing egg yolk as a cryoprotectant may pose hygienic risks and are difficult to standardize. Although a new generation of semen diluents free of animal ingredients is available, egg yolk-containing extenders are still widely used for cryopreserving semen. We compared the effects of using different extenders on bovine sperm function in vitro and on fertility in vivo. A soy lecithin extender (SL; AndroMed) and an egg yolk-containing (TRIS-EY) extender were tested. No differences (P>0.05) were detected between the two extenders for sperm-zona pellucida binding capacity (HZI=115+/-13). Assessment of the inducibility of the acrosome reaction with progesterone showed no differences (P>0.05) between extenders for live acrosome-reacted sperm (15+/-2.36 and 14.42+/-2.02%, respectively, for SL and TRIS-EY). However, post-thaw sperm motility was significantly lower (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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