Screening of β-fructofuranosidase-producing microorganisms and effect of pH and temperature on enzymatic rate
| Autor: | Beatriz Guilarte Maresma, Cristiane Angélica Ottoni, Elda Sabino da Silva, Rosa Mitiko Saito Matsubara, Luis Roberto Magossi, Maria Alice Alves Wada, Maria Filomena de Andrade Rodrigues, José Marcio Carter, Rubén Cuervo Fernandez, Alfredo Eduardo Maiorano |
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| Rok vydání: | 2007 |
| Předmět: |
Aspergillus oryzae
Microorganism Oligosaccharides Applied Microbiology and Biotechnology Reaction rate Industrial Microbiology Hydrolysis Soil Microbiology chemistry.chemical_classification beta-Fructofuranosidase biology Strain (chemistry) Fructosyltransferase activity Aspergillus niger Fungi Temperature General Medicine Hydrogen-Ion Concentration biology.organism_classification Saccharum Enzyme Hexosyltransferases chemistry Biochemistry Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 75:87-93 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-006-0803-x |
| Popis: | Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of beta-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase activity higher than 12,500 units l(-1). In addition, the beta-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. The temperature and pH effects on the sucrose-beta-fructofuranosidase reaction rate were studied using a 2(2) factorial experimental design. The comparative analysis of the tested variable coefficients shows that the variable pH contributes mostly to the changes in the fructosyltransferase and hydrolytic rates and in the V (t)/V (h) ratio. At 40 and 50 degrees C, there were no significant differences between the fructosyltransferase and hydrolytic velocities of these enzymes. |
| Databáze: | OpenAIRE |
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