The biological activities of Arabis alpina L. subsp. brevifolia (DC.) Cullen against food pathogens
| Autor: | Neslihan Balpinar |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
antimicrobial activity Arabis alpina biology Chemistry food and beverages antioxidant activity hplc General Chemistry biology.organism_classification 010603 evolutionary biology 01 natural sciences food-borne pathogens arabis Arabis Botany Materials Chemistry Food microbiology QD1-999 010606 plant biology & botany |
| Zdroj: | Open Chemistry, Vol 16, Iss 1, Pp 930-936 (2018) |
| ISSN: | 2391-5420 |
| Popis: | In this study, in vitro biological activities of both methanol and ethanol extracts of Arabis alpina subsp. brevifolia were investigated. Also, the phenolic components of this plant was examined in this study. The extracts were tested against the eight strains of food pathogens for their antimicrobial activities by utilizing minimum inhibitory concentration (MIC) and disc diffusion assay. The non-enzymatic antioxidant activities were determined according to scavenging of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The phenolic compounds were analyzed by high performance liquid chromatography (HPLC). The main component was ellagic acid for the methanol extract of stem-leaf, rutin for the ethanol extract of stem-leaf, and 2,5-dihydroxybenzoic acid for the methanol and ethanol extracts of fruit-flower. The ethanolic extracts of leaves revealed antibacterial activities against Salmonella Typhimurium (7 mm) while the ethanolic extracts of flowers demonstrated no activity against the test pathogens. The methanolic extracts of leaf-flower showed antibacterial activities against S. Typhimurium (7 mm). No activity was observed against C. albicans. The MIC value for four test bacteria was 13000 μg/mL. The ethanol extracts of A. alpina subsp. brevifolia exhibited the highest DPPH inhibition (76%). This study showed that A. alpina subsp. brevifolia possesses antioxidant and antimicrobial activities. |
| Databáze: | OpenAIRE |
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