Neuroprotective effect of naringenin against MPTP-induced oxidative stress
| Autor: | Sathiya Sekar, Mani Sugumar, Murugan Sevanan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Glutathione reductase Excitotoxicity Nitric Oxide Synthase Type II Substantia nigra Pharmacology medicine.disease_cause Neuroprotection Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Dopamine medicine Animals Parkinson Disease Secondary Dose-Response Relationship Drug General Neuroscience MPTP Neurodegeneration General Medicine Catalase medicine.disease Corpus Striatum Substantia Nigra Oxidative Stress Glutathione Reductase Neuroprotective Agents 030104 developmental biology nervous system chemistry 1-Methyl-4-phenyl-1 2 3 6-tetrahydropyridine Flavanones Lipid Peroxidation 030217 neurology & neurosurgery Oxidative stress medicine.drug |
| Zdroj: | International Journal of Neuroscience. 129:534-539 |
| ISSN: | 1543-5245 0020-7454 |
| Popis: | Background Parkinson's disease is the most common neurodegenerative disorder, characterized by loss of dopaminergic neurons in substantia nigra and depletion of dopamine in striatum due to excitotoxicity, oxidative stress and many other factors may contribute to MPTP- and PD-related neurodegeneration. The present study deals with the neuroprotective effect of Naringenin (NGN), a bioflavonoid against MPTP-induced Parkinson's disease in the mouse model. Methods Healthy male C57BL/6J mice (18-22 g b wt) were pretreated with NGN [25, 50, 100 mg/kg/b.wt, p.o] once daily for 5 days. Thereafter, 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) (80 mg/kg b.wt, i.p) was given in two divided doses (2 × 40 mg/kg at 16 h interval). The animals were observed for motor functions 48 h after the first MPTP injection. After completion of behaviour tasks, all animals were euthanized to dissect out the brain and used for biochemical, molecular and histopathological investigations. Results Pretreatment of NGN significantly reversed the toxic effects of MPTP by reducing LPO levels and increasing the activities of glutathione reductase and catalase along with improved behavioural performance. Interestingly, pre-treatment with NGN down-regulated iNOS expression level in MPTP intoxicated mice brain. In addition, the histopathological evaluation revealed that NGN decreased the nuclear pigmentation and cytoplasmic vacuolation in the substantia nigra and striatal regions when compared to MPTP-intoxicated mice brain. Discussion The present study showed that NGN exerts neuroprotection by suppressing oxidative stress via antioxidant mechanisms. The above finding suggests that NGN may act as a potential target in the management of PD. |
| Databáze: | OpenAIRE |
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