Gut Microbiota Associated with Gestational Health Conditions in a Sample of Mexican Women
| Autor: | Tizziani Benítez-Guerrero, Juan Manuel Vélez-Ixta, Carmen Josefina Juárez-Castelán, Karina Corona-Cervantes, Alberto Piña-Escobedo, Helga Martínez-Corona, Amapola De Sales-Millán, Yair Cruz-Narváez, Carlos Yamel Gómez-Cruz, Tito Ramírez-Lozada, Gustavo Acosta-Altamirano, Mónica Sierra-Martínez, Paola Berenice Zárate-Segura, Jaime García-Mena |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Nutrition and Dietetics
Bacteria Fatty Acids Volatile Gastrointestinal Microbiome Diabetes Gestational Feces Pre-Eclampsia Pregnancy RNA Ribosomal 16S Humans Dysbiosis mother feces high-throughput DNA sequencing fecal microbiota gut microbiota gestational diabetes pre-eclampsia pre-gestational diabetes Bruker Solarix XR FT ICR MS Female Food Science |
| Zdroj: | Nutrients; Volume 14; Issue 22; Pages: 4818 |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu14224818 |
| Popis: | Gestational diabetes (GD), pre-gestational diabetes (PD), and pre-eclampsia (PE) are morbidities affecting gestational health which have been associated with dysbiosis of the mother’s gut microbiota. This study aimed to assess the extent of change in the gut microbiota diversity, short-chain fatty acids (SCFA) production, and fecal metabolites profile in a sample of Mexican women affected by these disorders. Fecal samples were collected from women with GD, PD, or PE in the third trimester of pregnancy, along with clinical and biochemical data. Gut microbiota was characterized by high-throughput DNA sequencing of V3-16S rRNA gene libraries; SCFA and metabolites were measured by High-Pressure Liquid Chromatography (HPLC) and (Fourier Transform Ion Cyclotron Mass Spectrometry (FT-ICR MS), respectively, in extracts prepared from feces. Although the results for fecal microbiota did not show statistically significant differences in alfa diversity for GD, PD, and PE concerning controls, there was a difference in beta diversity for GD versus CO, and a high abundance of Proteobacteria, followed by Firmicutes and Bacteroidota among gestational health conditions. DESeq2 analysis revealed bacterial genera associated with each health condition; the Spearman’s correlation analyses showed selected anthropometric, biochemical, dietary, and SCFA metadata associated with specific bacterial abundances, and although the HPLC did not show relevant differences in SCFA content among the studied groups, FT-ICR MS disclosed the presence of interesting metabolites of complex phenolic, valeric, arachidic, and caprylic acid nature. The major conclusion of our work is that GD, PD, and PE are associated with fecal bacterial microbiota profiles, with distinct predictive metagenomes. |
| Databáze: | OpenAIRE |
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