Parasite infectivity and immunity to Plasmodium falciparum gametocytes in Gambian children
| Autor: | Chris Drakeley, Wijnand Eling, J. T. Bousema, Robert Sauerwein, Geoffrey A. T. Targett, K.A.E.M. Teelen, Brian Greenwood |
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| Rok vydání: | 2004 |
| Předmět: |
Molecular Sequence Data
Plasmodium falciparum Immunology Antibodies Protozoan Antigens Protozoan Biology Serology Antigen Immunity medicine Gametocyte Animals Humans Amino Acid Sequence Malaria Falciparum Infectivity Infant medicine.disease biology.organism_classification Virology Culicidae Child Preschool Carrier State biology.protein Parasitology Microbial pathogenesis and host defense [UMCN 4.1] Antibody Peptides Malaria |
| Zdroj: | Parasite Immunology, 26, 159-65 Parasite Immunology, 26, 4, pp. 159-65 |
| ISSN: | 0141-9838 |
| Popis: | Contains fulltext : 59070.pdf (Publisher’s version ) (Closed access) Immunity to the sexual stages of Plasmodium falciparum can be induced during natural infections. Characterization of this immunity may facilitate the design of a transmission-blocking vaccine (TBV). This study aimed to assess the prevalence and serological correlates of functional transmission-blocking immunity in Gambian children (aged 1-4 years old) who were P. falciparum gametocyte carriers. Serological assays showed 100% response to fixed, whole parasites but only 42% to live gametes. Responses to the antigens Pfs230 and Pfs48/45 were 54.1% and 37.3%, respectively, in an IgG1 ELISA. 14/55 sera were capable of reducing the infectivity of laboratory isolate NF54 in a standard membrane-feeding assay (SMFA). This activity was strongly correlated with IgG1 responses to Pfs48/45 (r = 0.49, P < 0.001) and to a serological reaction with epitopes of the same molecule (r = 0.38, P = 0.003). A weaker correlation was observed with IgG1 to Pfs230 (r = 0.29, P = 0.03). In direct membrane feeding assays (DMFA) with autologous isolates, sera from 4/29 children showed transmission-blocking activity. There was no correlation with serological assays and the DMFA or between the SMFA and DMFA. This may be caused by variation in sexual stage antigens and/or alternative modes of transmission-blocking immunity, both of which have implications for vaccine implementation. |
| Databáze: | OpenAIRE |
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