Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples

Autor:	Daniel Toribio-Avedillo, Judit Martínez-González, Javier Méndez, Raquel Mangas-Casas
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0301 basic medicine Water microbiology Veterinary medicine Somatic cell 030106 microbiology lcsh:Medicine 010501 environmental sciences Biology 01 natural sciences Article Applied microbiology 03 medical and health sciences Microbiologia aquàtica Bacteriophages lcsh:Science 0105 earth and related environmental sciences Contaminació de l'aigua Multidisciplinary lcsh:R Liter Water analysis Water sample Virus Water quality Water pollution Viruses lcsh:Q Qualitat de l'aigua Anàlisi de l'aigua
Zdroj:	Scientific Reports, Vol 10, Iss 1, Pp 1-5 (2020) Dipòsit Digital de la UB Universidad de Barcelona Scientific Reports
ISSN:	2045-2322
DOI:	10.1038/s41598-020-60071-w
Popis:	Emerging water quality guidelines and regulations require the absence of somatic coliphages in 100 mL of water, yet the efficiency of standardized methods to test this volume of sample is questionable. A recently described procedure, Bluephage, using a modified E. coli host strain, overcomes some of the methodological limitations of standardized methods. In a maximum of 6.5 hours (2.5 hours for pre-growing the host strain and 4 hours for the presence/absence test), Bluephage allows the direct detection of one plaque-forming unit (PFU) in a 100 mL water sample. The test shows high levels of specificity for somatic coliphages and comparable accuracy with standardized methods.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b252a11bc3ea4d3672a28ce29cf5165d http://link.springer.com/article/10.1038/s41598-020-60071-w Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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