DNAzyme-controlled plasmonic coupling for SERS-based determination of Salmonella typhimurium using hybridization chain reaction self-assembled G-quadruplex

Autor:	Wanqing Xu, Xueqi Leng, Tong Jin, Su Liu, Yu Wang, Jiadong Huang
Rok vydání:	2022
Předmět:	G-Quadruplexes Salmonella typhimurium Limit of Detection Biosensing Techniques DNA Catalytic Analytical Chemistry
Zdroj:	Microchimica Acta. 189
ISSN:	1436-5073 0026-3672
DOI:	10.1007/s00604-021-05154-9
Popis:	A facile and rapid SERS strategy for S. typhimurium detection based on hybridization chain reaction (HCR) self-assembled G-quadruplex DNAzyme (GQH DNAzyme)-controlled plasmonic coupling was developed. GQH DNAzyme is introduced as a biocatalyst to catalyze the oxidation of L-cysteines to cysteines (thiols to disulfides) to assist SERS signal transduction. This is the first time that the self-assembled split GQH DNAzyme-controlled plasmonic coupling is integrated with SERS sensing. The results reveal the proposed SERS strategy can quantify S. typhimurium with a wide linear range (5 to 10
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b250ad44e61dc0e65cf472040ef8ad26 https://doi.org/10.1007/s00604-021-05154-9 Zobrazit plný text záznamu Full text from SpringerLink