| Autor: |
Jonathan Hall, Constance Ciaudo, Harry Wischnewski, Fionna E. Loughlin, von Schroetter C, Frédéric Ha, Ugo Pradere, Kazeeva T, Duszczyk Mm |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| DOI: |
10.1101/572156 |
| Popis: |
Dead End (DND1) is an RNA-binding protein essential for germline development through its role in post-transcriptional gene regulation. The molecular mechanisms behind selection and regulation of its targets are unknown. Here, we present the solution structure of DND1’s tandem RNA Recognition Motifs (RRMs) bound to AU-rich RNA. The structure reveals how an NYAYUNN element is specifically recognized, reconciling seemingly contradictory sequence motifs discovered in recent genome-wide studies. RRM1 acts as a main binding platform, including unusual extensions to the canonical RRM fold. RRM2 acts cooperatively with RRM1, capping the RNA using an unusual binding pocket, leading to an unprecedented mode of tandem RRM-RNA recognition. We show that the consensus motif is sufficient to mediate upregulation of a reporter gene in human cells and that this process depends not only on RNA binding by the RRMs, but also on DND1’s double-stranded RNA binding domain (dsRBD), that we show to be dispensable for target binding in cellulo . Our results point to a model where DND1 target selection is mediated by an uncanonical mode of AU-rich RNA recognition by the tandem RRMs and a role for the dsRBD in the recruitment of effector complexes responsible for target regulation. Author comment A previous version of this manuscript contained an error in the normalization of the luciferase activity in the cell-based assays in Figures 5 and S8. This has been corrected and the text has been updated accordingly. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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