Nucleotide sequence of the kanamycin resistance transposon Tn903
| Autor: | Atsuhiro Oka, Mituru Takanami, Hiroyuki Sugisaki |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Peptide Biosynthesis
Genetics Transposable element Kanamycin Resistance Base Sequence R Factors Deoxyribonucleotides Nucleic acid sequence Biology Molecular biology Composite transposon Kanamycin Structural Biology Simple transposon DNA Transposable Elements Escherichia coli Consensus sequence Insertion sequence Molecular Biology Sequence (medicine) |
| Zdroj: | Journal of Molecular Biology. 147:217-226 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(81)90438-1 |
| Popis: | The entire nucleotide sequence of the kanamycin resistance transposon Tn903 was determined by analyzing a mini-ColE1 derivative carrying Tn903. Tn903 was 3094 base-pairs in length and at both extremities possessed two identical inverted 1057 base-pair sequences. Furthermore, 18 bases at the ends of the 1057 base-pair sequence are themselves present in an invertedly repeated order as has been described for various insertion sequences. Analysis of initiation and termination codons in the Tn903 sequence indicated that Tn903 could possibly code for at least three high molecular weight polypeptides. One in the region between the two 1057 base-pair sequences is suggested to be the kanamycin resistance determinant (aminoglycoside 3′-phosphotransferase) from its location and size. The other polypeptides were located within the 1057 base-pair sequence and may be associated with transposition functions of Tn903. |
| Databáze: | OpenAIRE |
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