Hepatic expression of toll-like receptors 3, 4, and 9 in primary biliary cirrhosis and chronic hepatitis C
| Autor: | Henry C. Bodenheimer, Kavitha Gopal, Petros C. Benias, Neil D. Theise |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Gastroenterology Peripheral blood mononuclear cell Cholangiocyte Primary biliary cirrhosis Internal medicine medicine Humans skin and connective tissue diseases Receptor Aged Hepatology Liver Cirrhosis Biliary business.industry TLR9 Hepatitis C Hepatitis C Chronic Middle Aged medicine.disease digestive system diseases Toll-Like Receptor 3 Toll-Like Receptor 4 Toll-Like Receptor 9 Immunology TLR4 Immunohistochemistry Female business |
| Zdroj: | Clinics and Research in Hepatology and Gastroenterology. 36:448-454 |
| ISSN: | 2210-7401 |
| DOI: | 10.1016/j.clinre.2012.07.001 |
| Popis: | Toll-like receptors (TLRs) may play a role in the inflammatory patterns observed in primary biliary cirrhosis (PBC) and chronic hepatitis C (CHC). We investigated TLR 3, 4 and 9 expression in PBC and CHC using immunohistochemical staining.Patient biopsies of PBC (N=11) and CHC (N=15) were compared to disease free livers (n=7). The extent of TLR staining was assessed separately according to a semi-quantitative scale for hepatocytes, cholangiocytes and portal mononuclear cells (PMC).In hepatocytes, TLR4 expression was increased (PBC; P=0.019), as was TLR9 (PBC; P=0.006, CHC; P=0.001). Cholangiocyte expression of TLRs 4 and 3 was reduced in both PBC (TLR4; P0.0001, TLR3; P=0.006) and CHC (TLR4; P0.0001, TLR3; P=0.014). Cholangiocyte expression of TLR9 was elevated for both groups and was significant in CHC (P=0.0115). PMCs showed up-regulation of TLR9 in PBC (P=0.022) and CHC (P=0.0001), with almost no expression of TLR 3 or 4.In PBC and CHC, hepatocytes showed increased TLR 4 and 9 expression without change in TLR3. Cholangiocytes showed increased TLR9 expression as opposed to down-regulation of TLRs 3 and 4. PMCs in both diseases had significantly increased TLR 9 expression perhaps implicating TLR9 expression in chronic liver inflammation. |
| Databáze: | OpenAIRE |
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