A comprehensive CHO SWATH-MS spectral library for robust quantitative profiling of 10,000 proteins
| Autor: | Daniel Ng, Yuansheng Yang, Hwee Tong Tan, Kelly Tan, Stephen Tate, Wei Zhang, Lillian Chia-Yi Liu, Kae Hwan Sim, Xuezhi Bi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Statistics and Probability Data Descriptor Proteome Expression systems Computer science Proteomic analysis CHO Cells Computational biology Library and Information Sciences 01 natural sciences Quality by Design Education 03 medical and health sciences Cricetulus Animals Profiling (information science) Biomanufacturing lcsh:Science Gene Library 030304 developmental biology 0303 health sciences Downstream processing Mass spectrometry Proteomic Profiling Chinese hamster ovary cell 010401 analytical chemistry 0104 chemical sciences Computer Science Applications lcsh:Q Statistics Probability and Uncertainty Information Systems |
| Zdroj: | Scientific Data Scientific Data, Vol 7, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2052-4463 |
| DOI: | 10.1038/s41597-020-00594-z |
| Popis: | Sequential window acquisition of all theoretical fragment-ion spectra (SWATH) is a data-independent acquisition (DIA) strategy that requires a specific spectral library to generate unbiased and consistent quantitative data matrices of all peptides. SWATH-MS is a promising approach for in-depth proteomic profiling of Chinese hamster Ovary (CHO) cell lines, improving mechanistic understanding of process optimization, and real-time monitoring of process parameters in biologics R&D and manufacturing. However, no spectral library for CHO cells is publicly available. Here we present a comprehensive CHO global spectral library to measure the abundance of more than 10,000 proteins consisting of 199,102 identified peptides from a CHO-K1 cell proteome. The robustness, accuracy and consistency of the spectral library were validated for high confidence in protein identification and reproducible quantification in different CHO-derived cell lines, instrumental setups and downstream processing samples. The availability of a comprehensive SWATH CHO global spectral library will facilitate detailed characterization of upstream and downstream processes, as well as quality by design (QbD) in biomanufacturing. The data have been deposited to ProteomeXchange (PXD016047). Measurement(s) Proteomic Profile • database type spectral library Technology Type(s) mass spectrometry • SWATH MS protein profiling assay • computational modeling technique Sample Characteristic - Organism Cricetulus griseus Machine-accessible metadata file describing the reported data: 10.6084/m9.figshare.12480386 |
| Databáze: | OpenAIRE |
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