Histological analysis of dental pulp response in immature or mature teeth after extra-oral subcutaneous transplantation into mice dorsum
| Autor: | Yasunori Akiyama, Toru Nagao, Masaki J. Honda, Sho Tanaka, Keita Otake, Taku Futenma, Tatsuaki Ito, Yuta Okuwa, Taku Toriumi, Kenichi Kurita |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Molar
Pathology medicine.medical_specialty Root canal Biology Extracellular matrix Mice 03 medical and health sciences Subcutaneous Tissue 0302 clinical medicine stomatognathic system medicine Extracellular Animals Regeneration General Dentistry Dental Pulp Odontoblasts Regeneration (biology) 030206 dentistry Transplantation stomatognathic diseases medicine.anatomical_structure Pulp (tooth) Alkaline phosphatase Dental Pulp Cavity 030217 neurology & neurosurgery |
| Zdroj: | Journal of Oral Science. 63:184-190 |
| ISSN: | 1880-4926 1343-4934 |
| DOI: | 10.2334/josnusd.20-0611 |
| Popis: | Purpose The aim of this study was to assess the response of dental pulp associated with donor or host cells in the pulp chamber and root canal after extra-oral transplantation. Methods Wild type or green fluorescent protein (GFP) transgenic first molars from 3-week, 6-week, and 12-week mice were transplanted into the subcutaneous layer of GFP mice or wild type mice. The teeth were histologically and immunohistochemically examined at 5 weeks after transplantation. Results Blood vessels present in the original coronal pulp had anastomosed with those from the recipient tissue that had invaded the root canal. Two distinct eosin-stained extracellular matrices were observed in the pulp chamber and root canal. Acellular matrix composed of nestin-positive, odontoblast-like cells invaded from the outside and was seen in the root canal of 3-week teeth. Cellular matrix comprising alkaline phosphatase (ALP)-positive fibroblast-like cells appeared in the original coronal pulp. In the root canal of the 6-week and 12-week teeth, cellular extracellular matrix consisting of ALP-positive fibroblast-like cells had invaded the recipient tissue. Conclusion Dental pulp from immature teeth might be able to regenerate dentin-like tissue. This model could be useful in the development of an optimized vitalization treatment. |
| Databáze: | OpenAIRE |
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