Aggregates-Based Boronlectins with Pyrene as Fluorophore: Multichannel Discriminative Sensing of Monosaccharides and Their Applications
| Autor: | Guo-wen Xing, Xiao-tai Zhang, Shu Wang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Analyte Pyrenes Fluorophore 010405 organic chemistry Chemistry Monosaccharides Analytical chemistry 010402 general chemistry Sensitivity and Specificity 01 natural sciences Fluorescence Fluorescence spectroscopy 0104 chemical sciences chemistry.chemical_compound Spectrometry Fluorescence Discriminative model Dynamic light scattering Lectins Pyrene Monosaccharide General Materials Science Boron |
| Zdroj: | ACS Applied Materials & Interfaces. 8:12007-12017 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.6b01940 |
| Popis: | Four-channel fluorescence assay toward six monosaccharides was achieved by employing two novel pyrene-functionalized boronlectins with flexible diboronic acid as receptors. The effects of pH values and aging time on the sensor properties were thoroughly evaluated by UV-vis, fluorescence spectroscopy and dynamic light scattering. We find that the fluorescence relative ratios were highly correlated with analyte concentrations at μM level. The flexibility of the receptors was perceived as an indispensable factor to produce diverse fluorescence signals toward different monosaccharides. Most importantly, integration of four fluorescence channels derived from the two sensors enables an excellent discrimination for all tested monosaccharides at a certain concentration or a concentration range via linear discriminant analysis (LDA). It is proposed that the multiple flexible linkers in the boronlectins could increase their self-adaptive capacity for different analytes, and facilitate the formation of stable boronlectin-sugar aggregate assemblies. In addition, practical sensing of glucose in the simulative blood and urine was illustrated to be feasible in the presence of interferences at physiological concentrations. |
| Databáze: | OpenAIRE |
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