Analysis of Hepatitis G Virus/GB Virus C Quasispecies and Replication Sites in Human Subjects
| Autor: | Tomasz Laskus, Janusz Cianciara, Lian Fu Wang, Jorge Rakela, Marek Radkowski |
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| Rok vydání: | 1999 |
| Předmět: |
Base Sequence
Flaviviridae Biophysics RNA Single-strand conformation polymorphism Cell Biology Viral quasispecies Biology Virus Replication biology.organism_classification Biochemistry GB virus C Virology Molecular biology Virus Species Specificity Capsid Viral replication Humans 5' Untranslated Regions Molecular Biology DNA Primers |
| Zdroj: | Biochemical and Biophysical Research Communications. 258:296-299 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1999.0632 |
| Popis: | Although the hepatitis G virus is unlikely to be a primary hepatotropic virus, its replication sites remain unclear. Using highly strand-specific Tth-based reverse transcriptase PCR we searched for the presence of the viral RNA negative strand in various autopsy tissues in two patients who died of end-stage liver disease. In addition, amplified viral sequences were compared in the 5' untranslated and the putative capsid regions by the single-strand conformation polymorphism (SSCP). Negative strand HGV RNA was detected in bone marrow and spleen from both patients and in lymph node tissue from one. All amplified sequences from a given patient were identical when compared by SSCP and direct sequencing. This lack of difference in the composition of quasispecies recovered from various tissues suggests the presence of a single, common viral compartment in the infected host. |
| Databáze: | OpenAIRE |
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