Pharmacodynamic evaluation of the effects of oral melatonin on expression of the T-cell cytokines interleukin-2 and interferon gamma in the dog
| Autor: | Anna C. Peace, Todd M. Archer, Andrew J. Mackin, Santosh Kumar, Crystal M. Hoh, Robert W. Wills |
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| Rok vydání: | 2018 |
| Předmět: |
Interleukin 2
Male medicine.medical_specialty 040301 veterinary sciences medicine.medical_treatment T-Lymphocytes Administration Oral 0403 veterinary science Melatonin 03 medical and health sciences Interferon-gamma 0302 clinical medicine Immune system Dogs Internal medicine medicine Animals Interferon gamma Whole blood Pharmacology General Veterinary business.industry Reverse Transcriptase Polymerase Chain Reaction 04 agricultural and veterinary sciences Reverse transcription polymerase chain reaction Endocrinology Cytokine Pharmacodynamics Interleukin-2 Female business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of veterinary pharmacology and therapeutics. 42(3) |
| ISSN: | 1365-2885 |
| Popis: | Objective To determine the effects of exogenous melatonin administration on activated whole blood expression of the T-cell cytokines interleukin-2 (IL-2) and interferon gamma (INF-γ) in dogs. Animals Ten healthy dogs. Procedures Heparinized whole blood was collected from 10 dogs for analysis of cytokine expression before administration of melatonin (baseline). Each dog was then administered melatonin at a dosage of approximately 1 mg/kg, PO, q 12 hr for 14 days. On day 14, whole blood was again collected from each dog at the time points of trough (0 hr) and 6 hr postmelatonin administration to evaluate the effects of melatonin on cytokine expression. At all evaluated time points, analysis of activated whole blood expression of mRNA coding for both IL-2 and IFN-γ was performed using quantitative reverse transcription polymerase chain reaction to determine whether a difference existed for any time point. Blood melatonin concentrations were also measured at comparable time points. Results A statistical difference in the expression of either cytokine was not appreciated at any time point, despite attainment of expected blood concentrations of the drug. Conclusions and clinical relevance Results indicate that melatonin therapy does not significantly affect IL-2 or IFN-γ expression in healthy dogs. While melatonin is thought to have an effect on the immune system in dogs, it does not appear this effect is through altering T-cell IL-2 or IFN-γ expression. Further studies investigating the effects of melatonin on the immune system of dogs are warranted. |
| Databáze: | OpenAIRE |
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