Yamogenin in fenugreek inhibits lipid accumulation through the suppression of gene expression in fatty acid synthesis in hepatocytes
| Autor: | Hiroki Murakami, Nobuyuki Takahashi, Tsuyoshi Goto, Keiko Taketani, Taku Uemura, Shohei Hoshino, Nobuaki Tsuge, Sayaka Moriwaki, Toshihiko Narukami, Teruo Kawada |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Diosgenin Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound Mice medicine Animals Humans RNA Messenger Liver X receptor Molecular Biology Fatty acid synthesis Triglycerides Yamogenin Liver X Receptors chemistry.chemical_classification biology Organic Chemistry Fatty Acids Fatty acid General Medicine Hep G2 Cells Lipid Metabolism Orphan Nuclear Receptors Sterol Fatty acid synthase medicine.anatomical_structure Trigonella chemistry Gene Expression Regulation Hepatocyte biology.protein Hepatocytes Sterol Regulatory Element Binding Protein 1 Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 78(7) |
| ISSN: | 1347-6947 |
| Popis: | Yamogenin is a diastereomer of diosgenin, which we have identified as the compound responsible for the anti-hyperlipidemic effect of fenugreek. Here, we examined the effects of yamogenin on the accumulation of triacylglyceride (TG) in hepatocytes, because yamogenin is also contained in fenugreek. It was demonstrated that yamogenin also inhibited TG accumulation in HepG2 hepatocytes and suppressed the mRNA expression of fatty acid synthesis-related genes such as fatty acid synthase and sterol response element-binding protein-1c. Indeed, yamogenin also antagonized the activation of the liver X receptor (LXR) in luciferase ligand assay similar to diosgenin. However, yamogenin could not exert such effects in the presence of T0901713, a potent agonist of LXR. These findings indicate that the effects of yamogenin on TG accumulation would be weaker than those of diosgenin, suggesting that the structural difference between yamogenin and diosgenin would be important for the inhibition of LXR activation. |
| Databáze: | OpenAIRE |
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