Comprehensive Analysis of the Expression and Prognosis for Laminin Genes in Ovarian Cancer
| Autor: | Ping Yang, Bowen Diao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Cell Kaplan-Meier Estimate Drug resistance CD8-Positive T-Lymphocytes Carcinoma Ovarian Epithelial cancer prognosis Pathology and Forensic Medicine Metastasis Immune system Laminin Biomarkers Tumor medicine Humans metastasis Survival rate Original Research Ovarian Neoplasms biology business.industry Pathology and Oncology Archive Oncogenes General Medicine Prognosis medicine.disease bioinformatical analysis Up-Regulation Gene Expression Regulation Neoplastic medicine.anatomical_structure ovarian cancer Oncology Cancer research biology.protein Female Ovarian cancer business laminins CD8 |
| Zdroj: | Pathology and Oncology Research |
| ISSN: | 1532-2807 1219-4956 |
| Popis: | Background: Ovarian cancer (OC) patients have a low survival rate, most patients with OC are diagnosed with advanced metastases, and recurrence is common. Dysregulation of cell laminin interactions plays an important role in the development of various cancers. However, it is unclear whether key laminin subunits serve as biomarkers for diagnosis, prognosis, and treatment in patients. Methods: In this study, multiple public databases were used including cBioPortal, GEO, ONCOMINE, GEPIA, Human Protein Atlas, Kaplan-Meier Plotter, TIMER, and Metascape databases. We used the database to analyze the value of laminin expression, prognosis, immune cell infiltration and drug resistance in OC. Results: Our study shows that mRNA expression of LAMA5, LAMB3, and LAMC2 are overexpressed in OC when compared with normal ovarian tissues, and the protein overexpression of LAMA1/A3/A5, LAMB1/B2/B3/B4, and LAMC1/C2 were shown in the OC tissue. Elevated LAMA4, LAMB1, and LAMC1 mRNA positively correlated with worse overall survival (OS) and progression-free survival (PFS) in OC. Higher LAMC2 mRNA expression was associated with a better OS, and higher LAMA2 mRNA related to better PFS, while LAMA5 can be used to predict poor prognosis of late-stage OS and high-grade PFS. LAMB3 and LAMC2 also expressively related to platinum resistance status and can be used to distinguish patients with primary or secondary OC patients. The ROC analysis of laminins in OC sets indicates that LAMA2/A4/A5, LAMB1/B2/B3, and LAMC2 display high precision in differentiating between malignant tumor and nonneoplastic tissue. LAMA5 is significantly related to macrophages (r = 0.207, p=4.84e-06) and dendritic cells (r = 0.249, p=3.08e-08). LAMC1 was significantly correlated with CD8+ T cells (r=−0.233, p= 2.53E-07). Specifically, LAMA4 and LAMB1 might closely related to tumor purity. Conclusions: Our results suggest that LAMA5, LAMB3 and LAMC2 could be used as predictors of OC survival and diagnosis. Moreover, our study speculates that LAMA5 and LAMC1 are important oncogenes in OC. Our study also calls for attention to the role of LAMA4, LAMB4, and LAMC1 in immune response. |
| Databáze: | OpenAIRE |
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