Comparative Evaluation of Commercial Concentration Procedures for Human Intestinal Parasite Detection
| Autor: | Damien Costa, Denis Lemeteil, Loïc Favennec, Gilles Gargala, Romy Razakandrainibe, Jean Jacques Ballet |
|---|---|
| Přispěvatelé: | CHU Rouen, Normandie Université (NU), Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Immunité de l'infection et transplantations, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Appareil Digestif Environnement Nutrition (ADEN ), Université de Rouen Normandie (UNIROUEN) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Veterinary medicine Concentration Adolescent [SDV]Life Sciences [q-bio] 030106 microbiology Clinical Biochemistry Intestinal parasite Stool specimen medicine.disease_cause Specimen Handling Comparative evaluation Feces Young Adult 03 medical and health sciences 0302 clinical medicine medicine Helminth Animals Humans Helminths Parasite hosting Parasites 030212 general & internal medicine Intestinal Diseases Parasitic Protozoa Child Aged Aged 80 and over biology Clinical Laboratory Techniques Diagnostic Tests Routine Biochemistry (medical) Infant Middle Aged biology.organism_classification 3. Good health Parasite Detection Stool Child Preschool Parasitic Intestinal Diseases Female |
| Zdroj: | Laboratory medicine Laboratory medicine, American Society of Clinical Pathologists, 2019, 50 (3), pp.243-248. ⟨10.1093/labmed/lmy072⟩ |
| ISSN: | 0007-5027 |
| DOI: | 10.1093/labmed/lmy072⟩ |
| Popis: | BackgroundEffective stool concentration is essential in microscopically based diagnosis of human intestinal parasite infections.ObjectiveTo compare the performances of 4 concentration commercial kits and 1 homemade procedure in 96 clinical stool specimens that tested positive for the detection of 9 helminth and 8 protozoa parasites.MethodsThe presence or absence of parasite forms was microscopically determined under conditions of standard practice. Also, we established the accuracies, concentration factors, and extraction yields.ResultsNo difference was observed between procedures for preconcentration specimens that tested positive. However, for preconcentration specimens that tested negative, we discovered that the homemade procedure was the most effective, and 2 of the 4 commercial kits were discovered to be satisfactory for routine applications.ConclusionsFor all parasites, procedures with biphasic solvents exhibited higher performances than organic solvent-free procedures. For the first time, the effectiveness of commercial concentration kits has been evaluated on several common stool parasites, and the results suggest that improvement of commercial procedures is possible. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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