Effect of Nitrogen Source on Cyanophycin Synthesis in Synechocystis sp. Strain PCC 6308
| Autor: | Nancy H. Kolodny, Wendy Mercer, Kristin M. Klucevsek, Kerstyn Bryce, Deborah Park, Mary M. Allen, Julie Wright, Adele J. Wolfson, Lea Medeiros, Amy L. Lane, Juliette Petersen, Courtney Yuen, Sogole Moin, Deborah Bauer |
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| Rok vydání: | 2006 |
| Předmět: |
Nitrogen
Cyanophycin Physiology and Metabolism Nitrogen assimilation Synechocystis Biology Nitrate reductase biology.organism_classification Microbiology Oxidative Stress chemistry.chemical_compound Bacterial Proteins chemistry Biochemistry Nitrogen fixation Ammonium Cyanophycinase Molecular Biology Plant Proteins Heterocyst |
| Zdroj: | Journal of Bacteriology. 188:934-940 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Cyanobacteria are capable of growing on a number of different nitrogen sources. Nitrate, nitrite, ammonium, urea, several amino acids, atmospheric nitrogen, nucleosides, and bases all have been reported to be assimilated by some cyanobacteria, but ammonium appears to be assimilated preferentially (7, 17). Nitrate is probably the most abundant form of combined nitrogen available for assimilation by cyanobacteria (10). The nondiazotrophic cyanobacterium Synechocystis sp. strain PCC 6308 has been shown to metabolize nitrate, ammonium, and diamino acids (4) and to incorporate the nitrogen from these sources into necessary metabolites, as well as into the nitrogen storage material, cyanophycin granule polypeptide (cyanophycin). Nitrogen limitation in nondiazotrophic cyanobacteria leads to many different effects that have been described in a variety of strains (4, 30). Utilization of different nitrogen sources first requires their passage through the permeability barrier of the cytoplasmic membrane into the cyanobacterial cell. At low nitrate concentrations, the endergonic uptake of nitrate in freshwater cyanobacteria takes place through ABC-type transporters that exhibit high affinity for nitrate (27). Passive diffusion of nitrate takes place in Synecchococcus sp. strain PCC 7942 at nitrate concentrations above 1 mM (26). Ammonium at low concentrations ( |
| Databáze: | OpenAIRE |
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