Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth
| Autor: | Gyllyandeson de Araújo Delmondes, Edy Sousa de Brito, Francisco Ernani Alves Magalhães, Abolghasem Siyadatpanah, Débora Lima Sales, Miriam Rolón, Gerson Javier Torres Salazar, José Weverton Almeida-Bezerra, Priscilla Augusta de Sousa Fernandes, Jacqueline Cosmo Andrade Pinheiro, Maria Flaviana Bezerra Morais-Braga, Veeranoot Nissapatorn, Cathia Coronel, Maria Celeste Vega Gomez, Francisco Lucas Alves Batista, Henrique Douglas Melo Coutinho, Marta Regina Kerntopf, Paulo Riceli Vasconcelos Ribeiro, Maria de Lourdes Pereira, Josefa Carolaine Pereira da Silva, Jaime Ribeiro-Filho |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Antioxidant antioxidant Antiparasitic medicine.drug_class medicine.medical_treatment Virulence phenolic compounds RM1-950 Biochemistry Microbiology Terpene medicine Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics biology Traditional medicine Chemistry Euphorbiaceae Croton heliotropiifolius biology.organism_classification Leishmania braziliensis Infectious Diseases Phytochemical cytotoxicity Therapeutics. Pharmacology Leishmania infantum antifungal |
| Zdroj: | Antibiotics, Vol 10, Iss 1074, p 1074 (2021) Antibiotics Volume 10 Issue 9 |
| ISSN: | 2079-6382 |
| Popis: | Croton heliotropiifolius Kunth (Euphorbiaceae), whose occurrence has already been registered in the most varied Brazilian biomes, is commonly found in the Chapada do Araripe, Ceará. The species is traditionally used to treat fungal, parasitic, and degenerative diseases. This study investigated the chemical composition and pharmacological potential (antioxidant, antifungal, antiparasitic, and cytotoxic) of an aqueous extract obtained from the roots of C. heliotropiifolius. Following a qualitative phytochemical screening, the chemical constituents were identified by ultra-efficiency liquid chromatography coupled witha quadrupole/time-of-flight system (UPLC-QTOF). The antioxidant potential was verified by thin-layer chromatography (TLC). The direct and combined antifungal activity of the extract against opportunistic Candida strains was investigated using the microdilution method. The minimal fungicidal concentration (MFC) was determined by subculture, while the modulation of the morphological transition (fungal virulence) was evaluated by light microscopy. The in vitro antiparasitic activity was analyzed using epimastigotes of Trypanosoma cruzi and promastigotes of Leishmania braziliensis and Leishmania infantum, while cytotoxicity was determined in cultures of mouse fibroblasts. The phytochemical analysis identified the presence of acids, terpenes, flavonoids, lignans, and alkaloids. Among these constituents, the presence of polar and non-polar phenolic compounds with known antioxidant action was highlighted. While the extract showed clinically ineffective antifungal effects, it could enhance the effectiveness of fluconazole, in addition to inhibiting the morphological transition associated with increased virulence in Candida strains. Although the extract showed low cytotoxicity against fibroblasts, it also had weak antiparasitic effects. In conclusion, Croton heliotropiifolius is a source of natural products with antifungal and antioxidant potential. |
| Databáze: | OpenAIRE |
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