Urinary conjugated α-tocopheronolactone—a biomarker of oxidative stress in children with type 1 diabetes

Autor: Mehul T. Dattani, D. P. R. Muller, Gayatri Sharma, Kevin Mills, Sinead Bryan, Peter Hindmarsh, Stephen M. P. O'Riordan
Rok vydání: 2013
Předmět:
Male
Tandem mass spectrometry
BMI
body mass index
Metabolite
Free radicals
medicine.disease_cause
Biochemistry
ODFR
oxygen-derived free radical
chemistry.chemical_compound
Diabetes mellitus
Vitamin E
LC–MS
liquid chromatography–mass spectrometry
Sulfation
Child
Macrovascular disease
Molecular Structure
α-TL
α-tocopheronolactone
Original Contribution
Biomarker (medicine)
Female
α-TTP
α-tocopherol transport protein
medicine.medical_specialty
Adolescent
Urinary system
HbA1c
glycosylated hemoglobin
α-Tocopheronolactone
VLDL
very low density lipoprotein
Urinary conjugated vitamin E metabolites
Internal medicine
Physiology (medical)
medicine
Humans
Glucuronidation
Platelet activation
α-CEHC
α-carboxyethylhydroxychroman
Type 1 diabetes
Biomarker
ACN
acetonitrile
medicine.disease
α-Carboxyethylhydroxychroman
Diabetes Mellitus
Type 1
Endocrinology
chemistry
Oxidative stress
GC–MS
gas chromatography–mass spectrometry
MeOH
methanol
Biomarkers
Chromatography
Liquid
Zdroj: Free Radical Biology & Medicine
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2012.09.012
Popis: Increased oxidative stress has been implicated in both the onset and the progression of diabetes mellitus and its complications. The development of easy to measure biomarkers of oxidative stress would, therefore, help in determining in a prospective manner the impact of glycemic control on oxidative stress and macrovascular disease in patients with diabetes. We report the development and validation of a novel method to directly measure the urinary concentrations of the conjugated metabolites of vitamin E (α-tocopherol) and investigate whether the oxidized metabolite α-tocopheronolactone (α-TL) could be used as a biomarker of oxidative stress in children with type 1 diabetes. A novel method using liquid chromatography–tandem mass spectrometry was developed and used to measure directly and rapidly the urinary concentrations of the glucuronidated and sulfated metabolites of α-tocopherol in 32 young patients with type 1 diabetes and age-matched controls. The mean concentrations of the glucuronidated and sulfated conjugates of α-TL were all highly significantly increased in the children with type 1 diabetes (p
Highlights ► A novel method was developed for measuring urinary conjugated vitamin E metabolites. ► α-Tocopheronolactone concentrations are highly significantly increased in diabetes. ► α-Tocopheronolactone is a potential biomarker of oxidative stress.
Databáze: OpenAIRE
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