Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by Comet assay and Micronucleus test
| Autor: | Xian Bing Li, Shaoying Liu, XiaoHui Yin, GuoNian Zhu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Animal Use Alternatives
Insecticides Erythrocytes Health Toxicology and Mutagenesis Toad medicine.disease_cause Bufo bufo Toxicology Lethal Dose 50 chemistry.chemical_compound biology.animal Genetics medicine Toxicity Tests Acute Animals Bufo Micronuclei Chromosome-Defective Micronucleus Tests biology Methane sulfonate biology.organism_classification Molecular biology Acute toxicity Comet assay chemistry Chlorpyrifos Larva Micronucleus test Hepatocytes Comet Assay Genotoxicity Water Pollutants Chemical DNA Damage Mutagens |
| Zdroj: | Mutation research. 680(1-2) |
| ISSN: | 0027-5107 |
| Popis: | In the present study, the genotoxicity of chlorpyrifos was evaluated in the Chinese toad by using Comet assay and Micronucleus test (MN), as the potential tools for the assessment of genotoxicity. The first step was determined by the acute toxicity of chlorpyrifos. Tadpoles were exposed to the series of relatively high concentrations of chlorpyrifos for 96 h. LC(50) values at 24, 48, 72, and 96 h were 3.63, 1.17, 0.82, and 0.80 mgl(-1), respectively. Secondly, the Micronucleus test was used for detecting chromosome damage in Chinese toad tadpoles exposed to the sublethal concentrations of chlorpyrifos and methyl methane sulfonate (MMS), which indicated that they induced chromosomal lesion in erythrocytes of Bufo bufo gargarizans tadpoles. Thirdly, the significant (P < 0.05 concentration-dependent increase in DNA damage (as indicated by Tail DNA%, Tail length, Olive tail moment)) were observed in erythrocytes and liver cells of tadpoles exposed to the sublethal concentrations of chlorpyrifos and MMS by Comet assay. To our knowledge, this is the first report to describe the use of B. bufo gargarizans for genotoxicity assessment of chlorpyrifos. |
| Databáze: | OpenAIRE |
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