GC1 deletion prevents light-dependent arrestin translocation in mouse cone photoreceptor cells
| Autor: | Susan L. Semple-Rowland, Jason E. Coleman |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
endocrine system Cell type genetic structures Light Biological Transport Active Chromosomal translocation Dark Adaptation Receptors Cell Surface Biology Mice Retinal Rod Photoreceptor Cells Arrestin medicine Animals Transducin Fluorescent Antibody Technique Indirect Vision Ocular Genetics Mice Knockout Retina Cell biology Mice Inbred C57BL Protein Transport medicine.anatomical_structure Microscopy Fluorescence Guanylate Cyclase Retinal Cone Photoreceptor Cells Arrestin beta 2 Arrestin beta 1 sense organs Gene Deletion Visual phototransduction |
| Zdroj: | Investigative ophthalmologyvisual science. 46(1) |
| ISSN: | 0146-0404 |
| Popis: | PURPOSE. Light-driven translocation of phototransduction regulatory proteins between the inner and outer segments of photoreceptor cells plays a role in the adaptation of these cells to light. The purpose of this study was to examine the effects of the absence of guanylate cyclase 1 (GC1) on light-driven protein translocation in rod and cone cells. Both cell types express GC1, but differ in sensitivity, saturation, and response times to light. METHODS. Immunohistochemical techniques employing antibodies specific for cone and rod transducin (T) subunits and arrestins were used to examine light-driven translocation of these proteins in the retinas of wild-type and GC1 knockout (KO) mice. RESULTS. Translocation of cone arrestin from cone outer segments to the inner cell regions was disrupted in the absence of GC1, whereas translocation of arrestin and T in rods was not affected. Cone T did not translocate in wild-type and GC1 KO mice, but differed in its subcellular distribution in GC1 KO retina, remaining in the cone outer segment in light and in dark. CONCLUSIONS. These results suggest that multiple, independent pathways regulate the translocation of phototransduction proteins and that GC1, and presumably cGMP, are of key importance in signaling the translocation of cone arrestin. (Invest Ophthalmol Vis Sci. 2005;46:12‐16) DOI:10.1167/iovs.040691 R |
| Databáze: | OpenAIRE |
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