Cell-specific protein profiling in Arabidopsis thaliana trichomes: identification of trichome-located proteins involved in sulfur metabolism and detoxification
| Autor: | Berit Ebert, Wolfram Weckwerth, Ulrike Simon-Rosin, Daniela Zoeller, Joachim Fisahn, Mirko Glinski, Stefanie Wienkoop |
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| Rok vydání: | 2004 |
| Předmět: |
biology
Arabidopsis Proteins Gene Expression Profiling Saccharomyces cerevisiae Arabidopsis Sulfur metabolism Plant Science General Medicine Horticulture biology.organism_classification Plant cell Proteomics Models Biological Biochemistry Mass Spectrometry Plant Epidermis Gene expression profiling Gene Expression Regulation Plant Gene expression Databases Protein Shotgun proteomics Molecular Biology Sulfur |
| Zdroj: | Phytochemistry. 65:1641-1649 |
| ISSN: | 0031-9422 |
| Popis: | Metabolite, protein, and transcript analysis at the cellular level gives unparalleled insight into the complex roles tissues play in the plant system. However, while capillary electrophoresis and PCR amplification strategies make the profiling of metabolites and transcripts in specific cell types possible, the profiling of proteins in small samples represents a bottleneck. Here for the first time protein profiling has been achieved in a specific plant cell type: The application of specific cell sampling and shotgun peptide sequencing (nano LC/MS/MS) resulted in the identification of 63 unique proteins from pooled Arabidopsis trichome cells. A complete S-adenosylmethionine pathway cluster, two S-adenosylmethionine synthase isoforms, a glutathione S-conjugate translocator and other proteins involved in sulfur metabolism and detoxification are shown to be present in these cells, in agreement with previous work done at the level of trichome transcript analysis. The technology described here brings the simultaneous identification and localization of physiologically relevant cellular proteins within reach. |
| Databáze: | OpenAIRE |
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