Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods

Autor: Wan Sy, Lee Fh, Abdul Rahim R, Zulkifli Idrus, Rosfarizan Mohamad, Teck Chwen Loh, Hooi Ling Foo
Rok vydání: 2019
Předmět:
0301 basic medicine
solid-state fermentation
Article
Catalysis
lactobacillus plantarum
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
chemistry.chemical_compound
Biotransformation
Lactobacillales
Extracellular
Food science
Physical and Theoretical Chemistry
Cellulose
lcsh:QH301-705.5
Molecular Biology
extracellular hydrolytic enzymes
Spectroscopy
palm kernel cake
biomass
biology
Hydrolysis
Organic Chemistry
Phoeniceae
0402 animal and dairy science
food and beverages
04 agricultural and veterinary sciences
General Medicine
Biodegradation
biology.organism_classification
040201 dairy & animal science
Computer Science Applications
Lactic acid
Biodegradation
Environmental
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Solid-state fermentation
Fermentation
Proteolysis
Food Microbiology
biotransformation
Extracellular Space
Bacteria
Lactobacillus plantarum
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 20, p 4979 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 20
ISSN: 1422-0067
DOI: 10.3390/ijms20204979
Popis: Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, &beta
glucosidase, &beta
mannosidase, and &beta
mannanase activities. Therefore, extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities of seven Lactobacillus plantarum strains (a prominent species of LAB) isolated from Malaysian foods were compared in this study. The biotransformation of palm kernel cake (PKC) biomass mediated by selected L. plantarum strains was subsequently conducted. The results obtained in this study exhibited the studied L. plantarum strains produced versatile multi extracellular hydrolytic enzyme activities that were active from acidic to alkaline pH conditions. The highest total score of extracellular hydrolytic enzyme activities were recorded by L. plantarum RI11, L. plantarum RG11, and L. plantarum RG14. Therefore, they were selected for the subsequent biotransformation of PKC biomass via SSF. The hydrolytic enzyme activities of treated PKC extract were compared for each sampling interval. The scanning electron microscopy analyses revealed the formation of extracellular matrices around L. plantarum strains attached to the surface of PKC biomass during SSF, inferring that the investigated L. plantarum strains have the capability to grow on PKC biomass and perform synergistic secretions of various extracellular proteolytic, cellulolytic, and hemicellulolytic enzymes that were essential for the effective biodegradation of PKC. The substantial growth of selected L. plamtraum strains on PKC during SSF revealed the promising application of selected L. plantarum strains as a biotransformation agent for cellulosic biomass.
Databáze: OpenAIRE
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