Changes in the expression of the potassium channels TASK1, TASK3 and TRESK in a rat model of oral squamous cell carcinoma and their relation to malignancy
| Autor: | Juan C. Cavicchia, Patricia Kunda, Cristian Gabriel Acosta, Walther David Zavala, Mabel Fóscolo |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Potassium Channels BETA TUBULIN 3 CIENCIAS MÉDICAS Y DE LA SALUD medicine.medical_treatment 4-Nitroquinoline 1-oxide Down-Regulation Nerve Tissue Proteins Malignancy Ciencias Biológicas 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Potassium Channels Tandem Pore Domain Downregulation and upregulation Tubulin medicine Animals Humans General Dentistry Chemotherapy Taxane business.industry K2P CHANNELS 4-NITROQUINOLINE-1-OXIDE Patología 030206 dentistry Cell Biology General Medicine Bioquímica y Biología Molecular medicine.disease Potassium channel Rats Otras Ciencias Médicas Blot stomatognathic diseases Medicina Básica 030104 developmental biology Otorhinolaryngology chemistry ORAL CANCER Cancer research Carcinoma Squamous Cell Immunohistochemistry Mouth Neoplasms POTASSIUM CHANNEL business CIENCIAS NATURALES Y EXACTAS |
| Popis: | Objectives: Potassium channels have been proposed to promote cancer cell proliferation and metastases. Thus, we investigated the expression pattern of three 2-pore domain potassium channels (K2Ps) TASK1, TASK3 and TRESK in advanced oral squamous cell carcinoma (OSCC), the commonest oral malignancy. Design: We used 4-nitroquinoline-1-oxide (4-NQO) to induce high grade OSCC in male adult rats. We then used immunohistochemistry and Western blotting to study the distribution and expression pattern of TASK1, TASK3 and TRESK in normal versus cancerous tissue. We also examined the expression of β-tubulin III (β-tub3), a marker associated with resistance to taxane-based chemotherapy and poor patient prognosis, and its correlation with the K2Ps. Finally, we studied the expression of TASK1, TASK3 and TRESK in human samples of SCC of oral origin. Results: We found that TASK3 was significantly up-regulated whereas TASK1 and TRESK were both significantly down-regulated in advanced, poorly differentiated OSCC. Both, rat and human SCC showed a significant increase in the expression of β-tub3. Interestingly, the expression of the latter correlated positively and significantly with TASK3 and TRESK but not TASK1 in rat OSCC. Our initial results showed a similar pattern of up and down regulation and correlation with β-tub3 for these three K2Ps in human SCC. Conclusions: The changes in expression and the co-localization with a marker of resistance to taxanes like β-tub3 turn TASK1, TASK3 and TRESK into potentially new prognostic tools and possibly new therapeutic targets for OSCC. Fil: Zavala, Walther David. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina Fil: Foscolo, Mabel Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Kunda, Patricia Elena. Instituto Universitario de Ciencias Biomédicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cavicchia, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Acosta, Cristian Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina |
| Databáze: | OpenAIRE |
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