Dietary curcumin and capsaicin: Relationship with hepatic oxidative stress and apoptosis in rats fed a high fat diet
| Autor: | Matem Tunçdemir, Muhammed Seyithanoğlu, Hikmet Koçak, Yıldız Öner-İyidoğan, Canan Başaran-Küçükgergin, Sevda Tanrıkulu-Küçük, Ibrahim Sogut, Semra Doğru-Abbasoğlu |
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| Přispěvatelé: | İÜC, Cerrahpaşa Tıp Fakültesi, Temel Tıp Bilimleri Bölümü |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
030213 general clinical medicine medicine.medical_specialty Curcumin Medicine (miscellaneous) Apoptosis Oxidative phosphorylation medicine.disease_cause Diet High-Fat capsaicin General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Internal Medicine medicine Animals Pharmacology (medical) curcumin Genetics (clinical) fatty liver chemistry.chemical_classification Reactive oxygen species TUNEL assay Chemistry Fatty liver Anti-Inflammatory Agents Non-Steroidal apoptosis medicine.disease Rats Oxidative Stress Endocrinology high-fat diet Liver Capsaicin Reviews and References (medical) lipids (amino acids peptides and proteins) Oxidative stress |
| Popis: | Abbasoglu, Semra/0000-0003-3467-9763; Oner-Iyidogan, Yildiz/0000-0001-6956-8794; Tuncdemir, Matem/0000-0002-5300-4449; KOCAK, Hikmet/0000-0003-1720-9102 WOS:000484049900002 PubMed ID: 30993920 Background. Apoptosis plays a major role in fatty liver disease. High-fat diets are related to the onset of fatty liver disease and hepatic oxidant-antioxidant imbalance. Curcumin and capsaicin are somewhat beneficial in reducing hepatic triglycerides; this is most likely because they are known to downregulate reactive oxygen species (ROS) and apoptosis. Objectives. The aim of this study was to investigate the effects of curcumin and capsaicin on apoptosis through the oxidative effect in an animal model of fatty liver disease. Material and methods. Male Sprague-Dawley rats were fed a normal control diet, a high-fat diet (HFD; 60% of total calories from fat), a HFD+curcumin (1.5 g curcumin/kg HFD), a HFD+capsaicin (0.15 g capsaicin/kg HFD), or a HFD+curcumin+capsaicin (1.5 g curcumin and 0.15 g capsaicin/kg HFD). Liver lysate levels of BAX, Bcl-2 and caspase-3 were determined via immunoblotting. Caspase-3 activity was measured with a colorimetric caspase-3 measurement kit. Total antioxidant status (TAS) and total oxidant status (TOS) were assayed using commercial kits. The generation of hepatic ROS was measured with fluorimetry. Fragmentation of DNA was detected using the TUNEL method. Results. High-fat diet caused increased expression of BAX and caspase-3, as well as increased TOS and caspase-3 activity, but decreased expression of Bcl-2. HFD+curcumin+capsaicin caused decreased BAX, caspase-3, TOS, and ROS levels as compared to HFD, but increased TAS and Bcl-2. A HFD +curcumin+capsaicin also decreased the number of TUNEL-positive cells. Conclusions. These results suggest that supplementation with curcumin and capsaicin balances the hepatic oxidant-antioxidant status and may have a protective role in the apoptotic process in an HFD-induced fatty liver model. Research Fund of Istanbul University, TurkeyIstanbul University [55331, BEK-2017-24926] This work was supported by the Research Fund of Istanbul University, Turkey, project No. 55331 and BEK-2017-24926. |
| Databáze: | OpenAIRE |
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