Fast gradient separation by very high pressure liquid chromatography: Reproducibility of analytical data and influence of delay between successive runs
| Autor: | Lois Ann Beaver, Fabrice Gritti, Paul G. Stevenson, Georges Guiochon, Joseph J. Stankovich |
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| Rok vydání: | 2013 |
| Předmět: |
Thermal equilibrium
Reproducibility Chromatography Chemistry Elution Organic Chemistry Flow (psychology) Temperature Analytical chemistry Reproducibility of Results General Medicine Biochemistry High-performance liquid chromatography Analytical Chemistry Volumetric flow rate Physics::Fluid Dynamics Phase (matter) Pressure Constant (mathematics) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 1318:122-133 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2013.09.069 |
| Popis: | Five methods were used to implement fast gradient separations: constant flow rate, constant column-wall temperature, constant inlet pressure at moderate and high pressures (controlled by a pressure controller), and programmed flow constant pressure. For programmed flow constant pressure, the flow rates and gradient compositions are controlled using input into the method instead of the pressure controller. Minor fluctuations in the inlet pressure do not affect the mobile phase flow rate in programmed flow. The reproducibilities of the retention times, the response factors, and the eluted band width of six successive separations of the same sample (9 components) were measured with different equilibration times between 0 and 15 min. The influence of the length of the equilibration time on these reproducibilities is discussed. The results show that the average column temperature may increase from one separation to the next and that this contributes to fluctuation of the results. |
| Databáze: | OpenAIRE |
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