Determination of cefotaxime, desacetylcefotaxime, cefmenoxime and ceftizoxime in biological samples by fluorescence detection after separation by thin-layer chromatography
| Autor: | Marie-Laure Rondot-Dudragne, Bernadette Mandrou, Huguette Fabre, Marie-Dominique Blanchin |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Cefotaxime
medicine.drug_class Cephalosporin Biochemistry Analytical Chemistry Cefmenoxime chemistry.chemical_compound Aminothiazole Ceftizoxime Electrochemistry medicine Environmental Chemistry Humans Spectroscopy Detection limit Chromatography Chemistry Silica gel Thin-layer chromatography Cephalosporins Spectrometry Fluorescence Chromatography Thin Layer medicine.drug |
| Zdroj: | The Analyst. 113(6) |
| ISSN: | 0003-2654 |
| Popis: | Cephalosporins bearing an aminothiazole group conjugated with a methoxyimino group give a strong fluorescence on a silica gel plate with a fluorescence indicator after development with an acidic mobile phase. A sensitive thin-layer chromatographic method based on this property has been developed for the determination of cefotaxime, desacetylcefotaxime, cefmenoxime and ceftizoxime. The detection limits and reliability of the proposed procedure allow the method to be used for the determination of these cephalosporins in therapeutic monitoring. |
| Databáze: | OpenAIRE |
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