Transcriptional Analysis of PRRSV-Infected Porcine Dendritic Cell Response to Streptococcus suis Infection Reveals Up-Regulation of Inflammatory-Related Genes Expression

Autor: Claude Lachance, Mariela Segura, Marcelo Gottschalk, Yingchao Wang, Gael Auray, Carl A. Gagnon
Přispěvatelé: Université de Montréal. Faculté de médecine vétérinaire
Rok vydání: 2016
Předmět:
0301 basic medicine
Streptococcus suis
Swine
Microarrays
viruses
animal diseases
lcsh:Medicine
Gene Expression
Pathology and Laboratory Medicine
Monocytes
White Blood Cells
Animal Cells
Zoonoses
Medicine and Health Sciences
lcsh:Science
Immune Response
Cells
Cultured
Swine Diseases
Immunity
Cellular
Multidisciplinary
biology
Coinfection
virus diseases
respiratory system
3. Good health
Up-Regulation
Bioassays and Physiological Analysis
Infectious Diseases
Intracellular Pathogens
Cell Processes
Host-Pathogen Interactions
Disease Susceptibility
medicine.symptom
Inflammation Mediators
Cellular Types
Pathogens
Research Article
Immune Cells
030106 microbiology
Immunology
Porcine Reproductive and Respiratory Syndrome
Inflammation
Research and Analysis Methods
Microbiology
03 medical and health sciences
Immune system
Signs and Symptoms
Phagocytosis
Immunity
Diagnostic Medicine
Streptococcal Infections
Virology
medicine
Genetics
Animals
Porcine respiratory and reproductive syndrome virus
Antigen-presenting cell
Blood Cells
Intracellular parasite
lcsh:R
Biology and Life Sciences
Dendritic cell
Dendritic Cells
Cell Biology
Porcine reproductive and respiratory syndrome virus
biology.organism_classification
030104 developmental biology
Co-Infections
lcsh:Q
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 5, p e0156019 (2016)
PLOS ONE
ISSN: 1932-6203
Popis: The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important swine pathogens and often serves as an entry door for other viral or bacterial pathogens, of which Streptococcus suis is one of the most common. Pre-infection with PRRSV leads to exacerbated disease caused by S. suis infection. Very few studies have assessed the immunological mechanisms underlying this higher susceptibility. Since antigen presenting cells play a major role in the initiation of the immune response, the in vitro transcriptional response of bone marrow-derived dendritic cells (BMDCs) and monocytes in the context of PRRSV and S. suis co-infection was investigated. BMDCs were found to be more permissive than monocytes to PRRSV infection; S. suis phagocytosis by PRRSV-infected BMDCs was found to be impaired, whereas no effect was found on bacterial intracellular survival. Transcription profile analysis, with a major focus on inflammatory genes, following S. suis infection, with and without pre-infection with PRRSV, was then performed. While PRRSV pre-infection had little effect on monocytes response to S. suis infection, a significant expression of several pro-inflammatory molecules was observed in BMDCs pre-infected with PRRSV after a subsequent infection with S. suis. While an additive effect could be observed for CCL4, CCL14, CCL20, and IL-15, a distinct synergistic up-regulatory effect was observed for IL-6, CCL5 and TNF-α after co-infection. This increased pro-inflammatory response by DCs could participate in the exacerbation of the disease observed during PRRSV and S. suis co-infection.
Databáze: OpenAIRE
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