Quantitative Detection of Anti-SARS-CoV-2 Antibodies Using Indirect ELISA
| Autor: | Shuhong Luo, Kelly C Whittaker, Chih Yun Cho, Jianmin Fang, Jianhua Xu, Siwei Zhu, Ruo-Pan Huang, Xingqi Wang, Meng Wang, Shehuo Xie, Jie Feng, Xuedong Song, Andy S Huang |
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| Rok vydání: | 2021 |
| Předmět: |
S1 RBD
Science Clinical Biochemistry Population Enzyme-Linked Immunosorbent Assay Antibodies Viral Sensitivity and Specificity Virus Seroepidemiologic Studies Humans Medicine Seroprevalence education dried blood education.field_of_study Receiver operating characteristic biology SARS-CoV-2 iELISA business.industry Biochemistry (medical) Albumin COVID-19 Gold standard (test) Virology Immunoglobulin A Dried blood spot Immunoglobulin M Immunoglobulin G biology.protein serum/plasma Antibody business AcademicSubjects/MED00690 nucleocapsid protein |
| Zdroj: | Laboratory Medicine |
| ISSN: | 1943-7730 0007-5027 |
| Popis: | Objective Real-time reverse transcription-polymerase chain reaction is the gold standard for the diagnosis of COVID-19, but it is necessary to utilize other tests to determine the burden of the disease and the spread of the outbreak such as IgG-, IgM-, and IgA-based antibody detection using enzyme-linked immunosorbent assay (ELISA). Materials and Methods We developed an indirect ELISA assay to quantitatively measure the amount of COVID-19 IgG, IgM, and IgA antibodies present in patient serum, dried blood, and plasma. Results The population cutoff values for positivity were determined by receiver operating characteristic curves to be 1.23 U/mL, 23.09 U/mL, and 6.36 U/mL for IgG, IgM, and IgA, respectively. After albumin subtraction, the specificity remained >98% and the sensitivity was 95.72%, 83.47%, and 82.60%, respectively, for IgG, IgM, and IgA antibodies to the combined spike subunit 1 receptor binding domain and N proteins in serum. Plasma and dried blood spot specimens were also validated on this assay. Conclusion This assay may be used for determining the seroprevalence of SARS-CoV-2 in a population exposed to the virus or in vaccinated individuals. |
| Databáze: | OpenAIRE |
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