Measurement of xylanase activity with insoluble xylan substrate
| Autor: | T M Enari, M Nummi, J M Perrin, M L Niku-Paavola |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
animal structures
Glycoside Hydrolases macromolecular substances engineering.material Xylose Biochemistry chemistry.chemical_compound Hydrolysis Nephelometry and Turbidimetry Polysaccharides Organic chemistry Sugar Molecular Biology Trichoderma Endo-1 4-beta Xylanases Chromatography Pulp (paper) technology industry and agriculture food and beverages Substrate (chemistry) Cell Biology Xylan carbohydrates (lipids) Aspergillus Xylosidases chemistry engineering Xylanase Xylans Acid hydrolysis Research Article |
| Zdroj: | Nummi, M, Niku-Paavola, M-L, Perrin, J-M & Enari, T-M 1985, ' Measurement of xylanase activity with insoluble xylan substrate ', Biochemical Journal, vol. 226, no. 2, pp. 617-620 . https://doi.org/10.1042/bj2260617 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2260617 |
| Popis: | Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan. |
| Databáze: | OpenAIRE |
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