Cell Division Drives Epithelial Cell Rearrangements during Gastrulation in Chick
| Autor: | Joao Firmino, Didier Rocancourt, Jerome Gros, Mehdi Saadaoui, Chloe Moreau |
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| Přispěvatelé: | Département de Biologie du Développement et Cellules souches - Department of Developmental and Stem Cell Biology, Institut Pasteur [Paris] (IP), Cellules Souches et Développement, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Cellule Pasteur UPMC, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Pasteur [Paris] (IP), Research in the J.G. laboratory is funded by the Institut Pasteur, the CNRS, the Vallee Foundation Young Investigator Award, the Cercle FSER, a starting grant from the FRM, and an ERC starting Grant., We thank F. Schweisguth for insightful discussions, M. Yanniv, S. Tajbakhsh, F. Schweisguth, S. Hopyan, C. Tabin, and T. Lecuit for critical reading of the manuscript, H. Sang, F. Song, and A. Sherman for sharing the memGFP transgenic chicken eggs prior to publication (from the transgenic chicken facility of the Roslin Institute in Edinburgh, Scotland, supported by the Wellcome Trust), and J.Y. Tinevez for help with the FRAP analysis., Institut Pasteur [Paris], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Sorbonne Université (SU) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell division [SDV]Life Sciences [q-bio] Cell Cell Culture Techniques Chick Embryo Biology General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 0302 clinical medicine Live cell imaging Cell Movement medicine Animals Cytoskeleton Molecular Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology Epithelial polarity Embryogenesis Gastrulation Embryo Epithelial Cells Cell Biology Actomyosin Cell biology 030104 developmental biology medicine.anatomical_structure [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis Chickens 030217 neurology & neurosurgery Cell Division Developmental Biology |
| Zdroj: | Developmental Cell Developmental Cell, 2016, 36 (3), pp.249-261. ⟨10.1016/j.devcel.2016.01.007⟩ Developmental Cell, Elsevier, 2016, 36 (3), pp.249-261. ⟨10.1016/j.devcel.2016.01.007⟩ |
| ISSN: | 1534-5807 |
| Popis: | International audience; During early embryonic development, cells are organized as cohesive epithelial sheets that are continuously growing and remodeled without losing their integrity, giving rise to a wide array of tissue shapes. Here, using live imaging in chick embryo, we investigate how epithelial cells rearrange during gastrulation. We find that cell division is a major rearrangement driver that powers dramatic epithelial cell intercalation events. We show that these cell division-mediated intercalations, which represent the majority of epithelial rearrangements within the early embryo, are absolutely necessary for the spatial patterning of gastrulation movements. Furthermore, we demonstrate that these intercalation events result from overall low cortical actomyosin accumulation within the epithelial cells of the embryo, which enables dividing cells to remodel junctions in their vicinity. These findings uncover a role for cell division as coordinator of epithelial growth and remodeling that might underlie various developmental, homeostatic, or pathological processes in amniotes. |
| Databáze: | OpenAIRE |
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