Characterization of gene expression profiles and functional analysis of peptidoglycan recognition protein 2 from rock bream (Oplegnathus fasciatus)
| Autor: | Mun-Gyeong Kwon, Jee Youn Hwang, Ji-Min Jung, Chan-Il Park, Kwang-Min Choi, Jin-Sol Bae, Jung Soo Seo, Bo-Yeong Jee, Dong Hee Cho, Do-Hyung Kim, Min-Soo Joo, Seong Don Hwang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
0301 basic medicine Aquatic Science Microbiology Cell membrane Fish Diseases Random Allocation 03 medical and health sciences Streptococcal Infections Complementary DNA Gene expression medicine Animals Environmental Chemistry Streptococcus iniae Amino Acid Sequence Edwardsiella tarda Phylogeny Innate immune system biology Gene Expression Profiling Enterobacteriaceae Infections 04 agricultural and veterinary sciences General Medicine biology.organism_classification DNA Virus Infections Immunity Innate Iridoviridae Perciformes Open reading frame 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Oplegnathus fasciatus 040102 fisheries 0401 agriculture forestry and fisheries Carrier Proteins Sequence Alignment Bacteria |
| Zdroj: | Fish & Shellfish Immunology. 84:1068-1074 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2018.11.025 |
| Popis: | Peptidoglycan recognition protein 2 (PGRP2) is a Zn2+-dependent peptidase that plays important roles in binding to microbial components of the cell membrane, inducing phagocytosis and antimicrobial activity. Rock bream (Oplegnathus fasciatus) PGRP2 (RbPGRP2) was identified in the intestine by next generation sequencing (NGS) analysis. The open reading frame (ORF) the RbPGRP2 cDNA (470 amino acid residues) contains a peptidoglycan recognition protein domain (residues 300 to 446). Alignment analysis revealed that RbPGRP2 shares 37.6-53.5% overall sequence identity with the PGRP2s of other species. Phylogenetic analysis revealed that RbPGRP2 clustered together with PGRP2s from teleosts. In healthy rock bream, RbPGRP2 was found to be ubiquitously expressed in all of the examined tissues, especially in the liver. RbPGRP2 expression was significantly upregulated in all of the examined tissues of rock bream after infection with Edwardsiella piscicida, Streptococcus iniae and red sea bream iridovirus (RSIV) compared with the control. Purified rRbPGRP2 interactions with bacteria and inhibited the growth of bacteria in the presence of Zn2+. These results indicate that RbPGRP2 plays an important role in the innate immune response against bacterial infection. |
| Databáze: | OpenAIRE |
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