Single molecule Raman spectroscopic assay to detect transgene from GM plants
| Autor: | Ulhas S. Kadam, Burkhard Schulz, R. L. Chavhan, Joseph Irudayaraj |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Plant Transgene Arabidopsis Biophysics Computational biology Biology Spectrum Analysis Raman Target concentration Polymerase Chain Reaction Biochemistry 03 medical and health sciences symbols.namesake Human health Caulimovirus Transgenic lines Transgenes Promoter Regions Genetic Molecular Biology Cell Biology Surface-enhanced Raman spectroscopy Plants Genetically Modified Molecular biology Highly sensitive 030104 developmental biology Agrobacterium tumefaciens symbols Biological Assay Amino Acid Oxidoreductases Raman spectroscopy |
| Zdroj: | Analytical Biochemistry. 532:60-63 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2017.06.002 |
| Popis: | Substantial concerns have been raised for the safety of transgenics on human health and environment. Many organizations, consumer groups, and environmental agencies advocate for stringent regulations to avoid transgene products' contamination in food cycle or in nature. Here we demonstrate a novel approach using surface enhanced Raman spectroscopy (SERS) to detect and quantify transgene from GM plants. We show a highly sensitive and accurate quantification of transgene DNA from multiple transgenic lines of Arabidopsis. The assay allows us to detect and quantify the transgenes as low as 0.10 pg without need for PCR-amplification. This technology is relatively cheap, quick, simple, and suitable for detection at low target concentration. |
| Databáze: | OpenAIRE |
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