Effect of degradation products of iron-bioresorbable implants on the physiological behavior of macrophages in vitro
| Autor: | Marcos A. Madrid, Rosa M. Lozano Puerto, Mónica Alicia Fernández Lorenzo de Mele, Natalia Fagali, María Eugenia Fernández, Blanca Pérez Maceda |
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| Přispěvatelé: | Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Fagali, Natalia S. [0000-0001-5489-137X], Madrid, Marcos A. [0000-0003-2093-783X], Pérez-Maceda, B. T. [0000-0001-5922-9131], Lozano, R.M. [0000-0003-2762-6938], Fernández Lorenzo de Mele, M. [0000-0002-8093-7551], Fagali, Natalia S., Madrid, Marcos A., Pérez-Maceda, B. T., Lozano, R.M., Fernández Lorenzo de Mele, M. |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
insoluble degradation products Biophysics Motility Biocompatible Materials macrophage 02 engineering and technology Biochemistry Cell Line Biomaterials Metal Mice 03 medical and health sciences iron Cell Movement In vivo Absorbable Implants Animals Macrophage tracking cell Cell Death Chemistry Macrophages Metals and Alloys 021001 nanoscience & nanotechnology In vitro Staining Resorption degradable biomaterial 030104 developmental biology Solubility Chemistry (miscellaneous) visual_art visual_art.visual_art_medium Degradation (geology) 0210 nano-technology Cell Division |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1756-591X 1756-5901 |
| DOI: | 10.1039/d0mt00151a |
| Popis: | 24 p.-7 fig. (In memory of Dr Blanca Teresa Perez-Maceda whose lovely life, plenty of fortitude, unwavering strength, joy and spiritual values, we had the honour of sharing.) The degradation of bioresorbable metals in vivo changes the physicochemical properties in the environment of an implant, such as a stent in the artery wall, and may induce the alteration of the functions of the surrounding cells. The Fe-degradation, from bioresorbable stents, is a particularly intricate process because it leads to the release of soluble (SDP) and insoluble degradation products(IDP) of varied composition. Macrophages are involved in the resorption of the exogenous agents coming from degradation of these materials. In the present work an Fe0 ring, made with a pure Fe wire,in contact with macrophage cell cultures was used to simulate the behaviour of a biodegradable Fe-based implant in a biological environment. Non-invasive time-lapse optical microscopy was applied to obtain images of macrophages exposed to Fe-degradation products, without using staining to avoid distortions and artefacts. It was noticed that as metal degraded, the IDP formed in situ accumulated close to the Fe0 ring. In this zone, the macrophages showed a dynamic process of uptake of dark Fe-containing products, confirmed by SEM-EDX. These macrophages showed alterations in the morphology and decrease in the motility and viability. The inability of the macrophages to move and to degrade the engulfed products caused a long persistence of IDP in the zone closest to the metal. The deleterious effects of IDP accumulated close to the ring, were significantly worse than those observed in the experiments made with (1) concentrated salt solutions (Fe3+ salt 3 mM), with the same amount of precipitates but uniformly distributed in the well, and (2) diluted salt solutions (Fe3+ salt 1 mM) with mainly soluble species. The results were confirmed by standard staining protocols that revealed dead cells close to the Fe0 ring and oxidative stress in cells exposed to both soluble and insoluble species. CONICET (P-UE 22920170100100CO), ANPCyT (PICT 2015-0232, PICT 2016-1424), UNLP(11/I221), MICINN (MAT2011-29152-C02-02), MINECO (MAT2015-67750-C3-2-R), RTI2018-101506-B-C33. |
| Databáze: | OpenAIRE |
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