The inhibition of bovine xanthine oxidase activity by Hg2+ and other metal ions
| Autor: | Madhu Sudan Mondal, Samaresh Mitra |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Xanthine Oxidase Metal ions in aqueous solution Kinetics Inorganic chemistry Mercury Biochemistry Divalent Ion Inorganic Chemistry chemistry.chemical_compound Xanthine oxidase activity chemistry Metals Spectrophotometry Animals Cattle Female Xanthine oxidase Copper Nuclear chemistry |
| Zdroj: | Journal of Inorganic Biochemistry. 62:271-279 |
| ISSN: | 0162-0134 |
| Popis: | The inhibition of the activity of bovine xanthine oxidase (XO) by divalent mercury and other metal ions has been investigated by optical spectroscopy and stop-flow kinetic measurements. The study shows that Hg2+ ion completely inhibits the activity of XO, while other metal ions such as Zn2+, Mg2+, Co2+, and Ni2+ inhibit the activity only marginally (approximately 10%). The inhibition by the Hg2+ ion was found to be monophasic and noncompetitive with strong affinity for binding to XO. The pH-dependent study of the inhibition indicates that at least two ionizing groups of XO are involved in the binding of the Hg2+ ion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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