| Autor: |
Ziwei Zhang, Dan Qiao, Yali Zhang, Qian Chen, Yujun Chen, Yingjue Tang, Renye Que, Ying Chen, Lie Zheng, Yancheng Dai, Zhipeng Tang |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Molecular nutritionfood research. 66(5) |
| ISSN: |
1613-4133 |
| Popis: |
To investigate the role of endoplasmic reticulum stress (ERS)-induced autophagy in inflammatory bowel disease (IBD) and the intervention mechanism of Portulaca oleracea L. (POL) extract, a medicinal herb with anti-inflammatory, antioxidant, immune-regulating, and antitumor properties, in vitro and in vivo.An IL-10-deficient mouse model is used for in vivo experiments; a thapsigargin (Tg)-stimulated ERS model of human colonic mucosal epithelial cells (HIECs) is used for in vitro experiments. The levels of ERS-autophagy-related proteins are examined by immunofluorescence and Western blot. Cellular ultrastructure is assessed with transmission electron microscopy. POL extract promotes a healing effect on colitis by regulating ERS-autophagy through the protein kinase R-like endoplasmic reticulum kinase (PERK)-eukaryotic initiation factor 2α (eIF2α)/Beclin1-microtubule-associated protein light chain 3II (LC3II) pathway.Overall, the results of this study further confirm the anti-inflammatory mechanism and protective effect of POL extract and provide a new research avenue for the clinical treatment of IBD. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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