Cysteine metabolism and whole blood glutathione synthesis in septic pediatric patients
| Autor: | Christopher Duggan, Alfred M. Ajami, Sharon Collier, Jeremy Lyons, Samuel Nurko, Xiao-Ming Lu, John Thompson, Leticia Castillo, Astrid Rauh-Pfeiffer, Sudhir Borgonha, Martha A. Q. Curley, David Zurakowski, Yong Ming-Yu, Vernon R. Young |
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| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Adolescent Critical Care and Intensive Care Medicine Gas Chromatography-Mass Spectrometry law.invention chemistry.chemical_compound law Internal medicine Sepsis medicine Humans Cysteine Prospective Studies Prospective cohort study Child Cysteine metabolism Whole blood business.industry Infant Calorimetry Indirect Glutathione Metabolism Intensive care unit Pathophysiology Surgery Endocrinology chemistry Case-Control Studies Child Preschool business Energy Intake |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0090-3493 |
| Popis: | OBJECTIVE To investigate whole body in vivo cysteine kinetics and its relationship to whole blood glutathione (GSH) synthesis rates in septic, critically ill pediatric patients and controls. DESIGN Prospective cohort study. SETTING Multidisciplinary intensive care unit and pediatric inpatient units at a children's hospital. PATIENTS Ten septic pediatric patients and ten controls (children admitted to the hospital for elective surgery). INTERVENTIONS Septic patients (age, 31 months to 17 yrs) and controls (age, 24 months to 21 yrs) received a 6-hr primed, constant, intravenous tracer infusion of l-[1-13C]cysteine. Blood samples were obtained to determine isotopic enrichment of plasma cysteine and whole blood [1-13C]cysteinyl-glutathione by gas-chromatography mass spectrometric techniques. The plasma flux and oxidation rate of cysteine and the fractional and absolute synthesis rates of GSH were determined. Septic patients received variable protein and energy intake, as per routine clinical management, and controls were studied in the early postabsorptive state. MEASUREMENTS AND MAIN RESULTS Plasma cysteine fluxes were increased in the septic patients when compared with the controls (68.2 +/- 17.5 [sd] vs. 48.7 +/- 8.8 micromol x kg(-1) x hr(-1); p |
| Databáze: | OpenAIRE |
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