Protection against cisplatin-induced nephrotoxicity by Spirulina in rats
| Autor: | Mahmood Khan, Aruna Prayag, Periannan Kuppusamy, J. C. Shobha, Iyyapu Krishna Mohan, Vijay Kumar Kutala, Madireddy Umamaheswara Rao Naidu |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Cancer Research Antioxidant Cell Survival medicine.medical_treatment Pharmacology Kidney Toxicology Antioxidants Nephrotoxicity Superoxide dismutase Lipid peroxidation Food-Drug Interactions chemistry.chemical_compound Acetylglucosaminidase Spirulina Tumor Cells Cultured medicine Animals Humans Urea Pharmacology (medical) Rats Wistar chemistry.chemical_classification Spirulina (genus) Glutathione Peroxidase Creatinine biology Superoxide Dismutase Glutathione peroxidase Catalase Creatine biology.organism_classification Rats medicine.anatomical_structure Oncology Biochemistry chemistry biology.protein Female Kidney Diseases Lipid Peroxidation Cisplatin |
| Zdroj: | Cancer Chemotherapy and Pharmacology. 58:802-808 |
| ISSN: | 1432-0843 0344-5704 |
| DOI: | 10.1007/s00280-006-0231-8 |
| Popis: | Purpose: Cisplatin (CP)-induced nephrotoxicity is associated with the increased generation of reactive oxygen metabolites and lipid peroxidation in kidney, caused by the decreased levels of antioxidants and antioxidant enzymes. The purpose of this study was to evaluate the role of Spirulina, blue–green alga with antioxidant properties, in the protection of cisplatin-induced nephrotoxicity in rat. Methods: Rats were treated with CP (6 mg/kg bw, single dose, intraperitoneally). Spirulina (1,000 mg/kg) was administered orally for 8 days and CP treatment was given on day 4. Nephrotoxicity was assessed, 6 days after the CP treatment, by measuring plasma urea, creatinine, urinary N-acetyl-(d-glucose-aminidase) (β-NAG) and histopathology of kidney. Results: Rats treated with CP showed marked nephrotoxicity as evidenced from the significant elevation in plasma urea, creatinine and urinary β-NAG. Histological assessment revealed marked proximal tubular necrosis and extensive epithelial vacuolization in the kidney of CP-treated rats. Superoxide dismutase, catalase and glutathione peroxidase were decreased and lipid peroxidation was increased in kidney tissue. Pretreatment with Spirulina protected the rats from CP-induced nephrotoxicity. The rise in plasma urea, creatinine, urinary β-NAG, plasma and kidney tissue MDA and histomorphological changes were significantly attenuated by Spirulina. In vitro studies using human ovarian cancer cells revealed that Spirulina did not interfere with the cytotoxic effects of CP on tumor cells. Conclusions: In summary, Spirulina significantly protected the CP-induced nephrotoxicity through its antioxidant properties. |
| Databáze: | OpenAIRE |
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